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植物学报  2011 

利用离体叶片鉴定杨树耐盐潜力

DOI: 10.3724/SP.J.1259.2011.00302, PP. 302-310

Keywords: 离体叶片,Na+积累,杨树,耐盐性

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Abstract:

?以受体杨树107和转基因杨树18-1的一年生枝条为材料,采用Hoagland营养液水培方法,添加不同浓度的NaCl,检测二者植株生长以及叶中Na+和K+含量的变化。结果表明,在0.6%NaCl处理下18-1生根率明显高于107,生物量较大,叶片中Na+积累量是107的1.45倍左右。以107和18-1离体叶片为材料,NaCl处理下测定其生理活性指标,发现18-1叶盘的失绿速度和相对电导率都显著低于107叶盘。把离体叶片接种在改良MS培养基上,1.2%NaCl处理可使107叶盘几乎停止伸长,细胞大小不再增加;而18-1叶盘培养7天后伸长了近50%。以上结果表明利用离体叶片可以鉴定出不同基因型杨树的耐盐潜力。

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