全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
植物学报  2011 

锦葵科植物DNA条形码通用序列的筛选

DOI: 10.3724/SP.J.1259.2011.00276, PP. 276-284

Keywords: DNA条形码,鉴定,ITS,ITS2,锦葵科,psbA-trnH

Full-Text   Cite this paper   Add to My Lib

Abstract:

?对锦葵科植物样品的ITS、ITS2、rbcL、matK和psbA-trnH序列进行PCR扩增和测序,比较各序列的扩增效率、测序成功率、种内和种间变异的差异以及barcodinggap图,使用BLAST1和NearestDistance方法评价不同序列的鉴定能力,进而从这些候选序列中筛选出较适合锦葵科植物鉴别的DNA条形码序列。结果表明,ITS序列在采集的锦葵科植物11个种26个样品中的扩增成功率较高,其种内、种间变异差异和barcodinggap较ITS2、psbA-trnH及rbcL序列具有更明显的优势,且纳入60个属316个种共1228个样品的网上数据后,其鉴定成功率可达89.9%。psbA-trnH序列的扩增和测序成功率最高,其鉴定成功率为63.2%,并能鉴别一些ITS序列无法鉴别的种。实验结果表明,ITS和psbA-trnH是较适合鉴别锦葵科植物的DNA条形码序列组合。

References

[1]  侯宽昭(1982).中国种子植物科属词典[M].北京:科学出版社.pp.293.
[2]  Pennisi E (2007). Wanted: a barcode for plants. Science 318(5848), 190—191.
[3]  Chen S L, Yao H, Han J P, et al. (2010). Validation of the ITS2 Region as a Novel DNA Barcode for Identifying Medicinal Plant Species. PLoS ONE 5(1), e8613.
[4]  Keller A, Schleicher T, Schultz J, et al. (2009). 5.8S-28S rRNA interaction and HMM-based ITS2 annotation. Gene 430(1-2), 50—57
[5]  罗焜,陈士林,陈科力等 (2010). 基于芸香科的植物通用DNA条形码研究[J]. 中国科学: 生命科学 40, 342-351.
[6]  朱英杰,陈士林,姚辉等 (2010). 重楼属药用植物DNA条形码鉴定研究[J]. 药学学报, 45, 376-382.
[7]  Lahaye R, Bank M, Bogarin D, et al. (2008). DNA barcoding the floras of biodiversity hotspots[J]. Proc Natl Acad Sci USA, 105(8), 2923-2928.
[8]  Slabbinck B, Dawyndt P, Martens M, et al. (2008). TaxonGap: a visualization tool for intra- and inter-species variation among individual biomarkers. Bioinformatics 24(6), 866-867.
[9]  THOMAS C (2009). Plant Bar Code Soon to Become Reality[J]. Science, 325(5940), 526.
[10]  Yao H, Song J Y, Ma X Y, et al. (2009). Identification of Dendrobium species by a candidate DNA barcode sequence: The chloroplast psbA-trnH intergenic region. Planta Med 75(6), 667-669.
[11]  Fazekas AJ, Burgess KS, Kesanakurti PR (2008). Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well[J]. PLoS one 3(7), e2802.
[12]  刘震,陈科力,罗焜等 (2010). 忍冬科植物DNA条形码通用序列的筛选. 中国中药杂志 35(17), 60-65.
[13]  CBOL Plant Working Group (2009). A DNA barcode for land plants. Proc Natl Acad Sci USA 106(31), 12794-12797.
[14]  Hollingsworth PM (2008). DNA barcoding plants in biodiversity hot spots: Progress and outstanding questions. Heredity 101, 1–2.
[15]  Sass C, Little D P, Stevenson D W, et al. (2007). DNA Barcoding in the Cycadales: Testing the potential of proposed barcoding markers for species identification of Cycads. PLOS ONE 2(11), e1154.
[16]  Newmaster SG, Fazekas AJ, Steeves RAD, Janovec J (2008). Testing candidate plant barcode regions in the Myristicaceae. Molecular Ecology Resources 8, 480–490.
[17]  宁淑萍, 颜海飞, 郝刚等 (2008). 植物DNA条形码研究进展. 生物多样性 16(5), 417—425.
[18]  Hebert P D, Cywinska A, Ball S L, et al. (2003). Biological identifications through DNA barcodes. Proc R Soc Biol Sci SerB 270(1512), 313—321.
[19]  Hebert P D, Ratnasingham S, deWaard J R (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc R Soc Biol Sci SerB 270(Supp 1), S96—S99.
[20]  Vences M, Thomas M, Bonett R M, et al. (2005). Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philos Trans R Soc Lond B Biol Sci 360(1462), 1859—1868.
[21]  Janzen D H, Hajibabaei M, Burns J M, et al. (2005). Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding. Philos Trans R Soc Lond B Biol Sci 360(1462), 1835—1845.
[22]  Ward R D, Zemlak T S, Innes B H, et al. (2005). DNA barcoding Australia's fish species. Philos Trans R Soc Lond B Biol Sci 360(1462), 1847—1857.
[23]  Kress W J, Wurdack K J, Zimmer E A, et al. (2005). Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci USA 102(23), 8369—8374.
[24]  Newmaster S G, Fazekas A J, Ragupathy S (2006). DNA barcoding in land plants: evaluation of rbcL in a multigene tiered approach. Can J Bot 84(3), 335—341.
[25]  Chase M W, Cowan R S, Hollingsworth P M, et al. (2007). A proposal for a standardised protocol to barcode all land plants. Taxon 56(2), 295—299.
[26]  Kress W J, Erickson D L (2007). A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2(6), e508.
[27]  Song J Y, Yao H, Li Y, et al. (2009). Authentication of the family Polygonaceae in Chinese pharmacopoeia by DNA barcoding technique. J Ethnopharmacol 124(3), 434-439.
[28]  陈士林, 宋经元, 姚辉等 (2009). 药用植物DNA条形码鉴定策略及关键技术分析. 中国天然药物 7(5), 322—327.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133