全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
植物学报  2011 

水稻蛋白质组双向电泳优化流程及方法

DOI: 10.3724/SP.J.1259.2011.00067, PP. 67-73

Keywords: 蛋白质组学,水稻,双向电泳

Full-Text   Cite this paper   Add to My Lib

Abstract:

?双向电泳是分析蛋白质混合物的一种有力手段,已在蛋白质组研究中得到广泛应用。水稻(Oryzasativa)作为重要的粮食作物,对其蛋白质组学研究开展较早。但由于技术复杂,对实验操作要求高,初学的研究者很难在较短的时间内掌握该实验技术。该文介绍了水稻研究中适合多个组织的双向电泳实验方法和优化流程。该优化流程能使新的研究者逐步优化实验条件,更快更好地完成双向电泳实验。同时详细介绍了实验关键环节的操作方法。

References

[1]  Lin YZ, Chen HY, Kao R, Chang SP, Chang SJ, Lai EM (2008) Proteomic analysis of rice defense response induced by probenazole. Phytochemistry 69, 715-728
[2]  Sengupta S, Majumder AL (2009) Insight into the salt tolerance factors of a wild halophytic rice, Porteresia coarctata: a physiological and proteomic approach. Planta 229, 911-929
[3]  Wei Z, Hu W, Lin Q, Cheng X, Tong M, Zhu L, Chen R, He G (2009) Understanding rice plant resistance to the Brown Planthopper (Nilaparvata lugens): a proteomic approach. Proteomics 9, 2798-2808
[4]  Yan S, Tang Z, Su W, Sun W (2005) Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics 5, 235-244
[5]  Yang P, Li X, Wang X, Chen H, Chen F, Shen S (2007) Proteomic analysis of rice (Oryza sativa) seeds during germination. Proteomics 7, 3358-3368
[6]  Yang Q, Wang Y, Zhang J, Shi W, Qian C, Peng X (2007) Identification of aluminum-responsive proteins in rice roots by a proteomic approach: cysteine synthase as a key player in Al response. Proteomics 7, 737-749
[7]  Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3 Edition edn. Los Banos: International Rice Research Institute, The Philippines
[8]  丁坤善, 郑彩霞, 包仁艳, 姜春宁 (2005) 油松雌性不育系球果蛋白质双向电泳. 植物学通报 22, 190-197
[9]  付忠军, 丁冬, 进茜宁, 王长城, 李永亮, 汤继华 (2009) 玉米花丝蛋白质组双向电泳条件的优化. 植物生理学通讯 45, 1215-1220
[10]  王清, 产祝龙, 秦国政, 田世平 (2009) 果实蛋白质组学研究的实验方法. 植物学报 44, 107-116
[11]  Ali GM, Komatsu S (2006) Proteomic analysis of rice leaf sheath during drought stress. Journal of proteome research 5, 396-403
[12]  Bokhari SA, Wan XY, Yang YW, Zhou L, Tang WL, Liu JY (2007) Proteomic response of rice seedling leaves to elevated CO2 levels. Journal of proteome research 6, 4624-4633
[13]  Ge C, Wan D, Wang Z, Ding Y, Wang Y, Shang Q, Ma F, Luo S (2008) A proteomic analysis of rice seedlings responding to 1,2,4-trichlorobenzene stress. J Environ Sci (China) 20, 309-319
[14]  Hashimoto M, Komatsu S (2007) Proteomic analysis of rice seedlings during cold stress. Proteomics 7, 1293-1302
[15]  He H, Li J (2008) Proteomic analysis of phosphoproteins regulated by abscisic acid in rice leaves. Biochem Biophys Res Commun 371, 883-888
[16]  Jagadish SV, Muthurajan R, Oane R, Wheeler TR, Heuer S, Bennett J, Craufurd PQ (2010) Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). Journal of experimental botany 61, 143-156
[17]  Kim ST, Kim SG, Hwang DH, Kang SY, Kim HJ, Lee BH, Lee JJ, Kang KY (2004) Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4, 3569-3578
[18]  Lee DG, Ahsan N, Lee SH, Kang KY, Bahk JD, Lee IJ, Lee BH (2007) A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics 7, 3369-3383
[19]  Lin SK, Chang MC, Tsai YG, Lur HS (2005) Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics 5, 2140-2156

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133