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植物学报  2012 

一个具有S位点的水稻类受体激酶OsSRL参与干旱胁迫反应

DOI: 10.3724/SP.J.1259.2012.00474, PP. 474-482

Keywords: 干旱胁迫,类受体激酶,水稻,RNA干涉,S-位点

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Abstract:

?植物在进化过程中针对干旱、高盐和高低温等逆境胁迫形成了多种适应机制,植物类受体激酶作为重要的细胞信号传递分子在植物生长和抗逆境胁迫中发挥着重要功能。该文发现一个具有S位点的类受体激酶基因OsSRL可能参与水稻(Oryzasativa)的干旱胁迫反应。利用RNAi技术降低OsSRL的表达水平后,转基因植株抗旱性增强,并表现出幼苗存活率、叶绿素含量及鲜重增加等表型。进一步的研究表明30%PEG和100μmol·L–1ABA可诱导OsSRL基因表达,利用RNAi降低其表达导致干旱诱导基因RAB16A及LEA3表达水平明显增加。表达模式分析发现OsSRL在胚芽、胚根、根、茎节以及花中表达。以上结果表明,OsSRL表达水平的降低增强植物的干旱耐受性,其作为一个S-位点样类受体激酶可能参与了水稻对干旱胁迫的反应。

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