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植物学报  2012 

甘蓝型油菜雌性不育突变体FS-M1乳突细胞的细胞学观察

DOI: 10.3724/SP.J.1259.2012.00036, PP. 36-43

Keywords: 甘蓝型油菜,雌性不育,细胞器,乳突细胞

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Abstract:

?雌性不育突变体FS-M1是从甘蓝型油菜(Brassicanapus)品种宁油10号中发现的。为了从细胞学角度研究FS-M1的雌性不育机理,利用荧光显微镜、扫描和透射电子显微镜观察分析了FS-M1柱头乳突细胞的授粉行为和超微结构。结果表明:花粉粒能在FS-M1乳突细胞上附着和萌发形成花粉管,但花粉管无法穿越柱头乳突细胞;开花后的FS-M1乳突细胞迅速衰退而呈干瘪萎蔫状,在衰退过程中,FS-M1柱头乳突细胞的细胞器数量减少,细胞液泡化明显,高尔基体、内质网和线粒体等一些细胞器结构被逐渐破坏。因此,推测FS-M1的雌性不育性是由于柱头乳突细胞发育异常造成的。

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