全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

相关文章

更多...

不同培肥模式对茶园土壤微生物活性和群落结构的影响

DOI: 10.11674／zwyf.2013.0111, PP. 97-105

Keywords: 茶园土,施肥,土壤酶,土壤微生物群落结构,磷脂脂肪酸

Full-Text   Cite this paper   Add to My Lib

Abstract:

以闽东地区红黄壤茶园定位实验地为对象，通过测定6种不同施肥处理土壤微生物学特性，研究不同培肥对土壤微生物特性和生物化学过程的影响，阐明各指标间的相互关系。结果表明，除了单施无机肥处理外，半量化肥+半量有机肥、全量有机肥、全量化肥+豆科绿肥以及半量化肥+半量有机肥+豆科绿肥等的培肥方式均不同程度提高了土壤有机质，可培养微生物数量，微生物量碳、氮含量及土壤酶活性，尤以半量无机肥+半量有机肥+豆科牧草的培肥模式增幅更为明显，而单施无机肥不利于微生物的生长、酶活性的提高和维持生态系统的稳定性。微生物群落磷脂脂肪酸（PLFAs）标记主成分分析显示，各种不同施肥方式使微生物群落结构发生改变。相关分析表明，微生物量与可培养微生物数量、微生物磷脂脂肪酸含量之间的相关性明显高于微生物量与各种酶活性之间的相关性，说明微生物数量大小对微生物群落结构的影响大于对酶活性功能的影响。研究也表明土壤各微生物指标能从不同方面反映土壤肥力水平，所以采用各种不同的方法能更客观地评价闽东地区茶园红黄壤质量的优劣。

 References

[1]  Cao H, Yang H, Sun B, et al. Changes of microbial biomass and enzyme activities in garden soil as influenced by planting time[J]. soils, 2002(4): 197-200.
[2]  Frankenberger W T, Dick W A. Relationship between enzyme activities and microbial growth and activity indices in soil[J]. Soil Sci. Soc. Am. J, 1983, 47(5): 945-951.
[3]  Gray D B, Mary K T, Franis R, et al. Microbial and biochemical soil quality indicators and their potential for differentiating areas under contrasting agricultural management regimes[J]. Soil Biol. Biochem., 2004, 36(11): 1785-1792.
[4]  陈国潮. 土壤微生物量测定方法现状及其在红壤上的应用[J]. 土壤通报，1999，30(6)： 284-287.
[5]  Chen G C. Situation of soil microbial biomass detection methods and its application in red soils[J]. Chin. J. Soil Sci., 1999, 30(6): 284-287.
[6]  Kenndy A C. Bacterial diversity in agroecosystems[J]. Agric. Ecosyst. Environ., 1999, 74(1-3): 65-76.
[7]  Lugato E, Berti A, Giardini L. Soil organic carbon (SOC) dynamics with and without residue incorporation in relation to different nitrogen fertilisation rates[J]. Geoderma, 2006, 135: 315-321.
[8]  徐华勤，肖润林，杨知建，等. 不同培肥措施对红壤茶园土壤微生物量碳的影响[J]. 生态学杂志，2007，26(7)：1009-1013.
[9]  Xu H Q, Xiao R L, Yang Z J, et al. Effects of different fertilization on red soil microbial biomass C in tea garden[J]. Chin. J. Ecosyst., 2007, 26(7): 1009-1013.
[10]  徐华勤，肖润林，宋同清，等. 稻草覆盖与间作三叶草对丘陵茶园土壤微生物群落功能的影响[J]. 生物多样性，2008，16(2)：166-174.
[11]  Xu H Q, Xiao R L, Song T Q, et al. Effects of mulching and intercropping on the functional diversity of soil microbial communities in tea plantations[J]. Biodiversity Science, 2008, 16(2): 166-174.
[12]  邓欣，谭济才，尹丽蓉，等. 不同茶园土壤微生物数量状况调查初报[J]. 茶叶通讯，2005，32(2)：7-9.
[13]  Deng X, Tan J C, Yin L R, et al. Investigation on the quantitative condition of soil microbes in different tea garden[J]. Tea Communication, 2005, 32(2): 7-9.
[14]  Lin X J, Huang D F, Li W H, et al. Effects of fertilization regimes on yield, nutrition accumulation of tea and soil fertility [J]. Chin. J. Eco-Agric., 2012, 20(2): 151-157.
[15]  王利民，林新坚，黄东风，等. 红黄壤茶园不同培肥模式的土壤理化效应[J]. 东北林业大学学报，2012，40(1)：1-5.
[16]  Wang L M, Lin X J, Huang D F, et al. Effect of different fertilization patterns on physicochemical properties of red-yellow soil in tea garden[J]. Journal of Northeast Forestry University, 2012, 40(1): 1-5.
[17]  关松荫. 土壤酶及其研究法[M]. 北京：中国农业出版社，1986.
[18]  Guan S Y. Soil enzyme and its research methods[M]. Beijing: Agricultural Press, 1986.
[19]  Vance E D, Brookes P C, Jenkinson D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biol. Biochem., 1987, 19(6): 703-707.
[20]  Trasar-Cepeda C, Camina F, Leiros M C, et al. An improved method to measure catalase activity in soils[J]. Soil Biol. Biochem., 1999, 31(3): 483-485.
[21]  Kandeler E, Gerber H. Short-term assay of soil urease activity using colorimetric determination of ammonium[J]. Biology and Fertility of Soils, 1988, 6(1): 68-72.
[22]  Tabatabai M A, Bremner J M. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity[J]. Soil Biol. Biochem., 1969, 1(4): 301-307.
[23]  Frosteg?rd A, Tunlid A, B??th E. Phospholipid fatty acid composition, biomass and activity of microbial communities from two soil types experimentally exposed to diferent heavy metals[J]. Appl. Environ. Microbiol., 1993, 59(11): 3605-3617.
[24]  Kourtev P S, Ehrenfeld J G, H?ggelom M. Exotic plant species alter the microbial community structure and function in the soil[J]. Ecology, 2002, 83(11): 3152-3166.
[25]  Cavigelli M A, Robertson G P, Klug M J. Fatty acid methyl ester (FAME) profiles as measures of soil microbial community structure[J]. Plant and Soil, 1995, 170(1): 99-113.
[26]  Zelles L, Bai Q Y, Beck T, et al. Signature fatty acids in phospholipids and lipopolysaccharides as indicators of microbial biomass and community structure in agricultural soils[J]. Soil Biol. Biochem., 1992, 24(4): 317-323.
[27]  He J Z, Zheng Y, Chen C R, et al, Microbial composition and diversity of an upland red soil under long-term fertilization treatments as revealed by culture-dependent and culture-independent approaches[J]. J. Soils Sediments, 2008, 8(5): 349-358.
[28]  Girvan M S, Bullimore J, Pretty J N, et al. Soil type is the primary determinant of the composition of the total and active bacterial communities in arable soils[J]. Appl. Environ. Microbiol. , 2003, 69(3): 1800-1809.
[29]  Sessitsch A, Weilharter A, Gerzabek MH, et al. Microbial population structures in soil particle size fractions of a long-term fertilizer field experiment[J]. Appl. Environ. Microbiol., 2001, 67(9): 4215-4224.
[30]  单武雄，罗文，肖润林，等. 连续5年施菜籽饼肥和稻草覆盖对茶园土壤生态系统的影响[J].中国生态农业学报，2010，18(3)：472-476.
[31]  Shan W X, Luo W, Xiao R L, et al. Effect of 5-year rapeseed cake fertilization and straw mulching on tea plantation soil ecosystem[J]. Chin. J. Eco-Agric., 2010, 18(3): 472-476.
[32]  彭萍，李品武，杨水平，等. 施肥对茶园土壤微生物及土壤肥力的影响[J]. 西南农业学报，2006，19(6)：1096-1099.
[33]  Peng P, Li P W, Yang S P, et al. Effects of fertilization on tea garden edaphon and soil fertility[J]. Southwest China J. Agric. Sci., 2006, 19(6): 1096-1099.
[34]  高云超，朱文珊，陈文新. 土壤微生物生物量周转的估算[J]. 生态学杂志，1993，12(6)：6-10.
[35]  Gao Y C, Zhu W S, Chen W X. Estimation for biomass and turnover of soil microorganisms[J]. Chinese Journal of Ecology, 1993, 12(6): 6-10.
[36]  Maly S, Kralovec J, Hampel D. Effects of long-term mineral fertilization on microbial biomass, microbial activity, and the presence of r- and K-strategists in soil[J]. Biol. Fertil. Soils, 2009, 45(7): 753-760.
[37]  Ladd J N, Amato M, Li-Kai Z, et al. Differential effects of rotation, plant residue and nitrogen fertilizer on microbial biomass and organic matter in an Australian Alfisol[J]. Soil Biol. Biochem., 1994, 26(7): 821-831.
[38]  Kautz T, Wirth S, Ellmer F. Microbial activity in a sandy arable soil is governed by the fertilization regime[J]. Eur. J. Soil Biol., 2004, 40(2): 87-94.
[39]  Aber J D. Nitrogen cycling and nitrogen saturation in temperate forest ecosystems[J]. Trends in Ecology and Evolution, 1992, 7(7): 220-224.
[40]  Brookes P C. The use of microbial parameters in monitoring soil pollution by heavy metals[J]. Biol. Fertil. Soils, 1995, 19(4): 269-279.
[41]  Yao H, He Z, Wilson M J, et al. Microbial biomass and community structure in a sequence of soils with increasing fertility and changing land use[J]. Microb. Ecol., 2000, 40(3): 223-237.
[42]  徐晶，陈婉华. 不同施肥处理对湖南红壤中微生物数量及酶活性的影响[J]. 土壤肥料，2003，(5)：8-11.
[43]  Xu J, Chen W H, Sun R L. Effects of different fertilization systems on amount of soil microorganism and enzyme activity in red soil of Hunan[J]. Soils and Fertilizers, 2003, (5): 8-11.
[44]  Giusquiani P L, Pagliai M, Gigliotti G, et al. Urban waste compost: effects on physical, chemical and biochemical soil properties[J]. J. Environ. Qual., 1995, 24(1): 175–182.
[45]  孙瑞莲，赵秉强，朱鲁生，等. 长期定位施肥对土壤酶活性的影响及其调控土壤肥力的作用[J]. 植物营养与肥料学报，2003，9(4)：406-410.
[46]  Sun R L, Zhao B Q, Zhu L S, et al. Effects of long-term fertilization on soil enzyme activities and its role in adjusting-controlling soil fertility[J]. Plant Nutr. Fert. Sci., 2003, 9(4): 406-410.
[47]  任全，单武雄，肖润林，等. 不同施肥措施对红壤丘陵茶园土壤酶活性及呼吸强度的影响[J].农业现代化研究，2007，28(4)：498-500.
[48]  Ren Q, Shan W X, Xiao R L, et al. Impact of Fertilizers on Soil Enzyme Activity and Intensity of Breathing of Tea Plantation in Red-soil Hilly Region[J]. Reas. Agric. Modern., 2007, 28(4): 498-500.
[49]  刘恩科，赵秉强，李秀英，等. 长期施肥对土壤微生物量及土壤酶活性的影响[J]. 植物生态学报，2008，32(1)：176-182.
[50]  Liu E K, Zhao B Q, Li X Y, et al. Biological properties and enzymatic activity of arable soils affected by long-term different fertilization systems[J]. Journal of Plant Ecology, 2008, 32(1): 176-182.
[51]  Frostegard A, Tunlid A, Baath E. Use and misuse of PLFA measurements in soils[J]. Soil Biol. Biochem., 2011, 43(8): 1621-1625.
[52]  于树，汪景宽，李双异. 应用PLFA方法分析长期不同施肥处理对玉米地土壤微生物群落结构的影响[J]. 生态学报，2008，28(9)：4221-4227.
[53]  Yu S, Wang J K, Li S Y. Effect of long-term fertilization on soil microbial community structure in corn field with the method of PLFA[J]. Acta Ecol. Sin., 2008, 28(9): 4221-4227.
[54]  白震，张明，宋斗妍，等. 不同施肥对农田黑土微生物群落的影响[J]. 生态学报，2008，28(7)：3244-3253.
[55]  Bai Z, Zhang M, Song D Y, et al. Effect of different fertilizaiton on microbial community in an arable mollisol [J]. Acta Ecol. Sin., 2008, 28(7): 3244-3253.
[56]  Vries F T, Hoffland E, Eekeren N, et al. Fungal/bacteria ratios in grasslands with contrasting nitrogen management[J]. Soil Biol. Biochem., 2006, 38(8): 2092-2103.
[57]  Guckert J B, Hood M A, White D C. Phospholipid, esterlinked fatty acid profile changes during nutrient deprivation of Vibrio cholerae: Increases in the trans/cis ratio and proportions of cyclopropyl fatty acids[J]. Appl. Environ. Microbiol., 1986, 52(4): 794-801.
[58]  徐建明，张甘霖，谢正苗，等. 土壤质量指标与评价[M]. 北京：科学出版社，2010. 178-184.
[59]  Xu J M, Zhang G L, Xie Z M, et al. Indices and assessment of soil quality[M]. Beijing: Science Press, 2010. 178-184.
[60]  曹慧，杨浩，孙波，等. 不同种植时间菜园土壤微生物生物量和酶活性变化特征[J]. 土壤，2002(4)：197-200.
[61]  林新坚，黄东风，李卫华，等. 施肥模式对茶叶产量、营养累积及土壤肥力的影响[J]. 中国生态农业学报，2012，20（2）：151-157.
[62]  Glover J D, Reganold J P, Andrews P K. Systematic method for rating soil quality of conventional, organic, and integrated apple orchards in Washington State[J]. Agriculture, Ecosystems and Environment, 2000, 80(1-2): 29-45.
[63]  张平究，李恋卿，潘根兴，等. 长期不同施肥下太湖地区黄泥土表土微生物碳氮量及基因多样性变化[J]. 生态学报，2004，24(12)：2818-2824.
[64]  Zhang P J, Li L Q, Pan G X, et al. Influence of long-term fertilizer management on topsoil microbial biomass and genetic diversity of a paddy soil from the Tai Lake region, China[J]. Acta Ecol. Sin., 2004, 24(12): 2818-2824.
[65]  徐阳春，沈其荣，冉炜. 长期免耕与施用有机肥对土壤微生物生物量碳、氮、磷的影响[J].土壤学报，2002，39(1)：89-96.
[66]  Xu Y C, Shen Q R, Ran W. Effects of zero-tiliage and application of manure on soil microbial biomass C, N and pafter sixteen years of cropping[J]. Acta Pedol. Sin., 2002, 39(1): 89-96.
[67]  来璐，郝明德，王永功. 黄土高原旱地长期轮作与施肥土壤微生物量磷的变化[J]. 植物营养与肥料学报，2004，10(5)：546-549.
[68]  Lai L, Hao M D, Wang Y G. Changes of long-term rotation and fertilization on soil microbial phosphorus under dryland in Loess Plateau[J]. Plant Nutr. Fert. Sci., 2005, 10(5): 546-549.
[69]  李东坡，武志杰，陈利军，等. 长期培肥黑土微生物量磷动态变化及影响因素[J]. 应用生态学报，2004，15(10)：1897-1902.
[70]  Li D P, Wu Z J, Chen L J, et al. Dynamics of microbial biomass P and its affecting factors in a long-term fertilized black soil[J]. Chin. J. Appl. Ecol., 2004, 15(10): 1897-1902.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133