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中国沙漠  2014 

水分胁迫下马铃薯SnRK2基因的表达模式与生理响应

DOI: 10.7522/j.issn.1000-694X.2013.00341

Keywords: 干旱胁迫,马铃薯(Solanumtuberosum),SnRK2,基因表达,生理响应

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Abstract:

以“大西洋”和“陇薯3号”两个品种盆栽苗为试验材料,研究了水分胁迫下马铃薯(Solanumtuberosum)StSnRK2基因家族的表达特征及其与植株生理指标变化的相关性。结果表明:在发生严重水分胁迫时,“陇薯3号”和“大西洋”的抗氧化酶活性、MDA含量、相对电导率和脯氨酸含量均较对照显著提高,但增长幅度在品种间存在差异;StSnRK2基因家族的8个成员的相对表达量在“大西洋”和“陇薯3号”之间也存在明显差异,StSnRK2.1、StSnRK2.2和StSnRK2.3在两个品种中均表现为水分胁迫后相对表达量显著高于对照的趋势;StSnRK2.7的相对表达量与对照无显著差异,而StSnRK2.4在“陇薯3号”的相对表达量是对照的9.5倍,是8个基因成员在受水分胁迫后相对表达量最高的一个基因;StSnRK2.1、StSnRK2.2、StSnRK2.3、StSnRK2.4和StSnRK2.6的相对表达量均与部分生理指标呈极显著正相关,StSnRK2.5和StSnRK2.8两个基因的相对表达量与部分生理指标呈极显著负相关,StSnRK2.7基因的相对表达量与生理指标无显著相关性。

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