全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
科学通报  2014 

水稻条纹窄叶突变体nsl1的形态和生理分析及基因定位

DOI: 10.1360/csb2014-59-16-1508, PP. 1508-1515

Keywords: 水稻,条纹,窄叶,基因定位

Full-Text   Cite this paper   Add to My Lib

Abstract:

水稻(OryzasativaL.)叶形和叶色直接影响光能利用,最终影响其产量和品质,是水稻重要的农艺性状.通过甲基磺酸乙酯诱变籼稻缙恢10号发现了1个遗传稳定的水稻条纹窄叶突变体,暂命名为nsl1.nsl1在苗期叶片呈浅白色,拔节期后出现平行于叶脉分布的白色条纹.而且其叶片显著窄于野生型缙恢10号.nsl1突变体的白色条纹部位细胞内部叶绿体严重解体,叶绿素含量显著下降.荧光参数F0,Fv/Fm,FPSⅡ,qP和ETR均显著低于野生型,光合效率显著降低.nsl1的叶形叶色及生理的变化最终引起nsl1突变体株型矮小和产量相关性状的明显减小.该条纹窄叶性状受一对单隐性核基因控制,被定位于第3染色体长臂InDel16与InDel12之间,物理距离为204kb,在该区域尚未发现与已报道的叶色或窄叶相类似的基因.本研究为NSL1基因克隆和功能分析奠定了良好基础.

References

[1]  2 汪得凯, 刘合芹, 李克磊, 等. 一个水稻窄叶突变体的鉴定和基因定位. 科学通报, 2009, 54: 360-365
[2]  3 Fujino K, Matsuda Y, Ozawa K, et al. NARROW LEAF7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics, 2008, 279: 499-507
[3]  9 李娜, 储黄伟, 文铁桥, 等. 水稻白色中脉Oswm突变体的遗传分析与基因定位. 上海农业学报, 2007, 23: 1-4
[4]  10 桑贤春, 徐芳芳, 凌英华, 等. 水稻条斑花叶突变体st(t)的鉴定与遗传定位. 作物学报, 2010, 36: 211-216
[5]  11 Lichtenthaler H K. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Meth Enzymol, 1987, 48: 350-382
[6]  12 何瑞锋, 丁毅, 余金洪, 等. 水稻温敏叶绿素突变体叶片超微结构的研究. 武汉植物学研究, 2001, 19: 1-5
[7]  16 Fambrini C A, Vecchia F D, Degl'Innocenti E, et al. Characterization of a pigment-deficient mutant of sunflower (Helianthus annuus L.) with abnormal chloroplast biogenesis, reduced PS Ⅱ activity and low endogenous level of abscisic acid. Plant Sci, 2004, 167: 79-89
[8]  18 Gothandam K M, Kim E S, Cho H, et al. OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis. Plant Mol Biol, 2005, 58: 421-433
[9]  1 Qi J, Qian Q, Bu Q, et al. Mutation of the rice Narrow leaf 1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiol, 2008, 147: 1947-1959
[10]  4 王峰, 唐彦强, 苗润隆, 等. 水稻窄叶白化突变体nul1的鉴定与基因定位. 科学通报, 2012, 57: 2066-2071
[11]  5 李红昌, 钱前, 王赟, 等. 水稻白穗突变体基因的鉴定和染色体定位. 科学通报, 2003, 48: 268-270
[12]  6 Kensuke K, Akiko M, Mitsuo N, et al. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 1997, 12: 1241-1250
[13]  7 Sugimoto H, Kusumi K, Noguchi K, et al. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 2007, 52: 512-527
[14]  8 Sugimoto H, Kusumi K, Tozawa Y, et al. The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 2004, 45: 985-996
[15]  13 McCouch R S, Kochert G, Yu Z H, et al. Molecular mapping of rice chromosomes. Theor Appl Genet, 1988, 76: 815-829
[16]  14 Luo Z K, Yang Z L, Zhong B Q, et al. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10 (t) in rice (Oryza sativa L.). Genome, 2007, 50: 811-817
[17]  15 Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 259: 597-607
[18]  17 许凤华, 程治军, 王久林, 等. 水稻白条纹叶Gws基因的精细定位与遗传分析. 作物学报, 2010, 36: 713-720
[19]  19 Kunst L, Browse J, Somerville C. Altered chloroplast structure and function in a mutant of Arabidopsis deficient in plastid glycerol-3-phosphate acyltransferase activity. Plant Physiol, 1989, 90: 846-853

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133