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科学通报  2013 

退火时间对二维DNA晶体自组装的影响

DOI: 10.1360/972012-1765, PP. 1450-1455

Keywords: DNA自组装,DNA分子瓦,黏性末端,退火时间,DNA二维晶体

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Abstract:

DNA自组装的过程,不仅需要合适的缓冲液,还需要适当的退火时间.本文利用DNA自组装技术,以反向平行双交叉(double-crossover,DX)DNA分子瓦(DNAtile)作为建筑模块,带有互补黏性末端(sticky-ends)的分子瓦依据Watson-Crick碱基互补配对原则配对连接,成功制备出二维晶体结构.比较不同退火时间下形成的DNA分子瓦结构,采用非变性聚丙烯酰胺凝胶电泳(non-denaturingPAGE)表征.结果表明,退火时间为2.5h时形成的分子瓦的结构较稳定.等量混合此条件下形成的不同分子瓦,分别从50,37和25℃退火至室温,利用原子力显微镜(AFM)对退火后的结构进行表征,结果表明,起始退火温度为37℃时得到的DNA二维晶体较平整.

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