罗非鱼无乳链球菌LAMP快速检测方法的建立
Development of loop-mediated isothermal amplification for detection of Streptococcus agalactiae from Tilapia

针对无乳链球菌高度保守的表面免疫相关蛋白基因，采用环介导等温扩增技术(LAMP)，设计4条特异性引物，优化反应体系，建立罗非鱼无乳链球菌快速诊断方法. 研究结果表明，LAMP方法检测灵敏度达到30 CFU·mL-1，且对2株罗非鱼源无乳链球菌均能扩增出特异性片段，而对14株非无乳链球菌均未扩增出相应片段. 建立的LAMP检测方法具有高度的特异性和灵敏性，反应在1 h内完成，为罗非鱼无乳链球菌病的快速诊断提供了一种重要的技术手段.
To develop a technique for rapid detection of Streptococcus agalactiae from Tilapia，the loop-mediated isothermal amplification(LAMP) was introduced to amplify the surface immunogenic protein(SIP) gene. The detection method was established by using optimum reaction system with four specific primers of the target gene. The results showed that the method had perfect specificity，and the sensitivity was 30 CFU·mL-1，as the DNA of 2 strains of Streptococcus agalactiae were able to be amplified，while the other 14 strains of non-Streptococcus agalactiae were not. The amplification process can be finished in 1 hour. The method provides a better choice for rapid detection of Streptococcus agalactiae