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棉花学报  2015 

雷蒙德氏棉DnaJ蛋白的生物信息学预测及盐胁迫下的表达分析

DOI: 10.11963/issn.1002-7807.201505002, PP. 391-400

Keywords: DnaJ蛋白,雷蒙德氏棉,旱地棉,克劳茨基棉,生物信息学,表达模式,差异表达基因

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Abstract:

DnaJ蛋白是一类很大的蛋白家族,是近年来研究较多的与胁迫反应等相关的蛋白。本文利用生物信息学手段,系统研究了雷蒙德氏棉(Gossypiumraimondii)DnaJ蛋白的数目、分类、染色体定位、亚细胞定位、进化以及在旱地棉(Gossypiumaridum)和克劳茨基棉(G.klotzschianum)盐胁迫下的表达模式。结果显示雷蒙德氏棉基因组有119个DnaJ蛋白,长度从73个氨基酸到2574个氨基酸不等;在13条染色体上不均匀分布,且具有不同的亚细胞定位;DnaJ结构域大致被分为9个小组,每个小组都有与拟南芥同源的基因;盐胁迫下,鉴定到旱地棉和克劳茨基棉共同差异表达的DnaJ基因3个,在旱地棉盐胁迫整个过程中差异表达的基因2个。雷蒙德氏棉DnaJ基因家族的鉴定和分析,将为进一步研究DnaJ基因家族的功能奠定一定的基础。

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