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棉花学报  2008 

棉花恢复系中含有26SrRNA序列的GH18Rorf392基因克隆

DOI: 1002-7807(2008)05-0323-07, PP. 323-329

Keywords: 棉花,恢复系,抑制差减杂交,细胞质雄性不育,育性恢复

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Abstract:

以Y18R×P30A杂交F1代自交材料播种后根据雄蕊形态学判断其育性,然后分别提取可育和不育材料现蕾3~5d的幼蕾总RNA。通过抑制差减杂交的方法获得来自棉花恢复系的EST序列之后,以该EST序列设计引物再做5’和3’RACE,从而获得其全长cDNA序列。由于该基因来源于陆地棉(Gossypiumhirsutum)恢复系Y18R,并且编码392个氨基酸的蛋白质,因此,将其命名为GH18Rorf392。该基因的5’端是一个全新的序列,3’端含有26SrRNA序列。该基因可能对研究棉花育性相关功能有帮助。

References

[1]  [18] AKAGI H, Nakamura A, Yokozeki-misono Y, et al.Positional cloning of the rice Rf-1 gene, a restorer of BT-type cytoplasmic male sterility that encodes a mitochondrial-targeting PPR protein[J].Theor Appl Genet, 2004, 108: 1449-1457.
[2]  [19] 徐秉芳. 核编码的育性恢复基因[J]. 植物生理学通讯, 2000, 36(6): 573-580.
[3]  [1] 郭旺珍,张天真.棉花胞质雄性不育育性恢复基因的RAPD-PCR标记筛选[J]. 科学通报, 1997, 42(24): 2645-2647.
[4]  [2] DIATCHENKO L, Chrislau Y E, Campbell A P, et al. Suppression subtractive hybridization: a method for generating differential regulated or tissue-specific cDNA probes and libraties [J]. Proc Natl Acad Sci USA, 1996, 93:6025-6030.
[5]  [3] KUANG W W, Thompson D A, Hoch R V, et al. Differential screening and suppression subtractive hybridization identified genes differentially expressed in an estrogen receptor-positive breast carcinoma cell line[J]. Nucleic Acids Research, 1998, 26:1116-1123.
[6]  [4] GURSKAYA N G, Diatchenko L, Chenchik A, et al. Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristste 13-acetate[J]. Anal Biochem, 1991, 197: 91-95.
[7]  [5] VON STEIN O D, Thies W G, Hofmann M. A high through put screening for rarely transcribed differentially expressed genes[J]. Nucleic Acids Research, 1997, 25: 2598-2602.
[8]  [6] WAN Ching-yi, Wilkin T A. A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.) [J]. Anal Biochem, 1994, 223:7-12.
[9]  [7] 张天真. 陆地棉1355A和104-7A不育系的细胞学研究[J]. 棉花学报, 1995, 7(2): 73-75.
[10]  [8] 姚长兵, 胡绍安, 王春英,等. 棉花细胞质雄性不育小孢子母细胞败育的细胞学研究[J].棉花学报, 1994, 6(增刊): 25-27.
[11]  [9] SCHNABLE P S, Wise R P. The molecular basis of cytoplasmic male sterility and fertility restoration[J]. Trends Plant Sci, 1999, 3: 175-180.
[12]  [10] CUI Xiang-qin, Wise R P, Schnable P S. The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize[J].Science, 1996, 272: 1334-1336.
[13]  [11] KOIZUKA N, Imai R, Fujimoto H, et al.Genetic characterization of a pentatricopeptide repeat protein gene, orf687 that restores fertility in the cytoplasmic male-sterile Kosena radish[J].Plant J, 2003, 34: 407-415.
[14]  [12] KOMORI T, Ohta S, Murai N, et al.Map-based cloning of a fertility restorer gene, Rf-1, in rice ( Oryza sativa L. ) [J].Plant J, 2004, 37: 315-325.
[15]  [13] BENTOLILA S, Alfonso A A, Hanson M R.A pentatricopeptide repeatcontaining gene restores fertility to cytoplasmic malesterile plants[J].Proc Natl Acad Sci USA, 2002, 99: 10887-10892.
[16]  [14] BROWN G G, Formanova N, Jin H, et al.The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats[J].Plant J, 2003, 35: 262-272.
[17]  [15] DESLOIRE S, Gherbi H, Laloui W, et al.Identification of the fertility restoratioin locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family[J].EMBO Rep, 2003, 4: 588-594.
[18]  [16] IMAI R, Koizuka N, Fujimoto H, et al.Delimitation of the fertility restorer locus Rfk1 to a 43-kb contig in kosena radish (Raphamus sativus L. ) [J].Mol Gen Genet, 2003, 269: 388-394.
[19]  [17] KAZAMA T, Toriyama K.A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic malesterile rice [J].FEBS Lett, 2003, 544: 99-102.

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