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棉花学报  2010 

棉花GhDIS2基因的克隆与酵母表达

DOI: 1002-7807(2010)02-0099-05, PP. 99-103

Keywords: 棉花纤维,GhDIS2,克隆,酵母

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Abstract:

棉花纤维由胚珠外被单个表皮细胞分化发育而成,其分化和伸长涉及细胞骨架的重排。本研究根据已报道的植物DISTORTED2基因,同源候选基因法克隆棉花GhDIS2。该基因包括975bpORF,编码324个氨基酸。qPCR分析表明,它在陆地棉TM-1不同来源的组织中均表达,于开花后12d的纤维中达到表达高峰,开花后18d表达开始下降,根和茎的表达量较低。Southern杂交结果表明,GhDIS2在四倍体棉花基因组中存在2个拷贝。克隆GhDIS2到酵母表达载体中,并转化到裂殖酵母中,诱导该基因过量表达,GhDIS2促使细胞扩大。初步推测,GhDIS2和其它植物DIS2功能相似,参与植物细胞肌动蛋白骨架的组装。

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