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棉花学报  2011 

棉花多重PCR技术及其对杂交棉纯度鉴定的初步研究

DOI: 1002-7807(2011)01-0022-06, PP. 22-27

Keywords: 棉花,SSR,多重PCR,纯度检测

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Abstract:

选取在湘杂棉系列品种间表现出多态性稳定的部分SSR引物,基于其扩增片段大小的不同来组合引物,进行棉花多重PCR扩增。结果表明,在与单一PCR扩增相同反应条件下,仅根据扩增片段大小不同的原则,可使80%的两重PCR组合获得正常扩增。利用两重PCR组合正常扩增的引物进行三重、四重PCR反应时,均可获得正常扩增的产物,在此基础上提出棉花上简化的多重PCR优化程序。并将多重PCR技术应用于杂交棉种子纯度检测,清楚地鉴定出母本种子混杂和其它杂交棉种子的混杂,从而为棉花杂交种的快速准确鉴定奠定了技术基础。

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