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棉花学报  2011 

临界生长温度锻炼对棉苗抗冷性的影响

DOI: 1002-7807(2011)01-0090-04, PP. 90-93

Keywords: 棉苗,临界生长温度,低温胁迫,脯氨酸,低温诱导蛋白,抗冷性

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Abstract:

研究了棉苗经过不同低温(12℃、14℃、16℃)锻炼7d后,对较长时间(7d)的低温(4℃)胁迫的耐受能力,并探讨了与其相关的可能机理。结果表明经不同低温锻炼7d后,所有棉花品种的幼苗脯氨酸含量都有不同程度的增加,其中12℃临界生长温度锻炼后增幅最明显;临界生长温度(12℃)下锻炼后的棉苗能耐受较长时间(7d)的低温(4℃)冷胁迫,其抗冷性比对照明显提高。采用SDS-PAGE对棉苗中的蛋白质进行分离,发现所有参试品种经适当的低温锻炼后在冷胁迫下蛋白质含量和种类都有明显的变化,而且都有低温诱导蛋白(即抗冻蛋白)产生,不同品种产生的低温诱导蛋白可能有所差异。

References

[1]  LEVIT J. Resistance of plants to environmental stress: chilling, freezing, and high-temperature stress[M]. New York, USA: Academic Press, 1980.
[2]  RYU S B, Costa A, Xin Z G, et al. Induction of cold hardiness by salt stress involves synthesis of cold-and abscisic acid-responsive proteins in potato[J]. Plant Cell Physiol, 1995, 36: 1245-1251.
[3]  简令成,卢存福,李积宏,等.适宜低温锻炼提高冷敏感植物玉米和番茄的抗冷性及其生理基础[J].作物学报,2005,31(8): 971-976. JIAN Ling-cheng, Lu Cun-fu, Li Ji-hong, et al. Increment of chilling tolerance and its physiological basis in chilling-sensitive corn sprouts and tomato seedlings after cold-hardening at optimum temperatures[J]. Acta Agron Sinica, 2005, 31(8): 971-976.
[4]  林善枝,张志毅,林元震.植物抗冻蛋白及抗冻分子改良[J]. 植物生理与分子生物学报,2004,30(3):251-256.LIN Shan-zhi, Zhang Zhi-yi, Lin Yuan-zhen. Research on improvement of antifreeze proteins and antifreeze molecules[J]. Journal of Plant Physiology and Molecular Biology, 2004, 30(3): 251-256.
[5]  刘祖祺,张石城. 植物抗性生理学[M].北京:中国农业出版社, 1994:39-46,369.LIU Zhu-qi, Zhang Shi-cheng. Plant resistant physiology[M]. Beijing: China Agriculture Press, 1994: 39-46, 369.
[6]  沙月霞,简桂良,肖崇刚,等.棉叶总蛋白提取及SDS-PAGE电泳的改良[J].棉花学报,2005,17(3):146-150.SHA Yue-xia, Jian Gui-liang, Xiao Chong-gang, et al. An improved method for extraction and SDS-PAGE of total protein in cotton leaves[J]. Cotton Science, 2005, 17(3): 146-150.
[7]  YOSHIDA Y, Kiyosue T, Nakashima K, et al. Regulation of levels of proline as an osmolyte in plants under water stress[J]. Plant Cell Physiol, 1997, 38: 1095-1102.
[8]  XIN Z G, Li P H. Relationship between proline and ABA in the induction of chilling tolerance in maize suspension-cultured cells[J]. Plant Physiol, 1993, 103: 607-613.
[9]  董合铸,孙龙华.不同抗寒性小麦品种的麦苗在冰冻—化冻后叶片细胞亚显微结构的变化[J].植物学报,1980,22(4):339-342.DONG He-zhu, Sun Long-hua. Ultrastructural changes in leaf cells of wheat varieties with different cold resistance after freezing-thawing of the plants[J]. Acta Botanica Sinica, 1980, 22, 9(4): 339-342.
[10]  李生泉,侯燕平,范月仙.不同抗冷级别的棉苗在低温处理后叶片细胞超微结构的变化[J].电子显微学报,1998,17(3): 231-236.LI Sheng-quan, Hou Yan-ping, Fan Yue-xian. Ultrastructural of the leaf cell of cotton varieties with different cold-resistant levels under low temperature[J]. Journal of Chinese Electron Microscopy Society, 1998, 17(3): 231-236 .
[11]  江 勇,魏令波,费云标,等.分离和鉴定沙冬青抗冻蛋白质[J].植物学报,1999,41(9):967-971.JIANG Yong, Wei Ling-bo, Fei Yun-biao, et al. Purification and identification of antifreeze proteins in Ammopiptanthus mongolicus[J]. Acta Botanica Sinica, 1999, 41(9): 967-971.
[12]  田 云,卢向阳,张海文.抗冻蛋白研究进展[J].中国生物工程杂志,2002,22(6):48-53.TIAN Yun, Lu Xiang-yang, Zhang Hai-wen. Advances in the antifreeze proteins[J]. Journal of Chinese Biotechnology, 2002, 22(6): 48-53.

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