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棉花学报  2012 

两种常用激素组合下棉花体细胞胚胎发生过程的组织学观察

DOI: 1002-7807(2012)02-0159-08, PP. 159-166

Keywords: 棉花,体细胞胚胎发生,组织学观察,植物生长调节剂

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Abstract:

MSB培养基添加两种常用植物激素组合,Indole-3-butyricacid(IBA)+kinetin(KT)和2,4-dichlorophenoxyaceticacid(2,4-D)+KT,分别命名为IK和DK处理用来诱导陆地棉体细胞胚胎发生。陆地棉品系YZ1下胚轴切段作为外植体在诱导培养基上培养4周后,继代到分化培养基上促进体细胞胚胎发生。结果表明,两种处理均有体细胞胚胎发生,其中IK处理上外植体嫁接33d后就可见体细胞胚出现,分化率高达97.6%,大多数外植体表现为体细胞胚较胚性愈伤早出现。DK处理中体细胞胚胎发生慢,体细胞胚都是经过明显的胚性愈伤发育而来,胚性愈伤分化率明显低于IK处理,仅为28.6%。此外,IK处理中的外植体在培养过程中大部分都出现了须根,而DK处理中则没有。两种处理中出现了两种不同的体细胞胚胎发生过程,组织学观察结果表明DK和IK处理中的体细胞胚分别主要起源于初生形成层和皮层。

References

[1]  DAVIDONIS G H, Hamilton R H. Plant regenerating from callus tissues of Gossypium hirsutum L.[J]. Plant Science Letters,1983, 32: 89-93.
[2]  SHOEMAKER R C, Couche L J, Galbraith D W. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.)[J]. Plant Cell Reports, 1986, 3: 178-181.
[3]  FINER J J, Smith R H. Initiation of callus and somatic embryos from explants of mature cotton(Gossypium klotzschianum Anderss)[J]. Plant Cell Reports, 1984, 3: 41-43.
[4]  TROLINDER N L, Goodin J R. Somatic embryogenesis and plant regeneration in Gossypium hirsutum L.[J]. Plant Cell Reports, 1987, 6: 231-234.
[5]  TROLINDER N L, Goodin J R. Somatic embryogenesis in cotton (Gossypium). I. Effects of explants and hormone regime[J]. Plant Cell Tissue and Organ Culture, 1988a, 12: 31-42.
[6]  TROLINDER N L, Goodin J R. Somatic embryogenesis in cotton.II. Requirements for embryo development and plant regeneration[J]. Plant Cell Tissue and Organ Culture, 1988b, 12: 43-53.
[7]  FINER J J. Plant regeneration from somatic embryogenic suspension cultures of cotton Gossypium hirsutum L.[J]. Plant Cell Reports, 1988, 8: 586-589.
[8]  GAWEL N J, Robacker C D. Genetic control of somatic embryogenesis in cotton petiole callus cultures[J]. Euphytica, 1990, 49: 249-253.
[9]  VOO K S, Rugh C L, Kamalay J C. Indirect somatic embryogenesis and plant recovery from cotton[J]. In vitro Cellular & Developmental Biology-Plant, 1991, 27: 117-124.
[10]  张献龙,孙济中,刘金兰.陆地棉体细胞胚胎发生与植株再生[J].遗传学报,1991,18(5):461-467. ZHANG Xian-long, Sun Ji-zhong, Liu Jin-lan. Somatic embryogenesis and plant regeneration in upland cotton[J]. Acta Genetic Sinica, 1991, 18(5): 461-467.
[11]  FIROOZABADY E, Deboer D L. Plant regeneration via somatic embryogenesis in many cultivars of cotton(Gossypium hirsutum L.)[J]. In Vitro Cellular & Developmental Biology-Plant, 1993, 29: 166-173.
[12]  SAKHANOKHO H F, Zipt A, Rajasekaran K, et al. Induction of highly embryogenic calli and plant regeneration in upland (Gossypium hirsutum L.) and Pima (Gossypium barbadense L.) cottons[J]. Crop Science, 2001, 41: 1235-1240.
[13]  RAJASEKARAN K, Sakhanokho H F, Zipt A, et al. Somatic embryo initiation and germination in diploid cotton (Gossypium arboreum L.)[J]. In Vitro Cellular & Developmental Biology-Plant, 2004, 40: 177-181.
[14]  WU J H, Zhang X L, Nie Y C, et al. Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of Chinese cotton[J]. In vitro Cellular & Developmental Biology-Plant, 2004, 40: 371-375.
[15]  SUN Y Q, Zhang X L,Guo X P, et al. Somatic embryogenesis and plant regeneration from different wild diploid cotton (Gossypium)species[J]. Plant Cell Reports, 2006, 25(4): 289-296.
[16]  贺雅婷, 曲延英, 孔庆平, 等.海岛棉愈伤组织诱导及分化的影响因素初探[J].分子植物育种, 2008, 6(3): 597-602. HE Ya-ting, Qu Yan-yin, Kong Qing-ping, et al. Primary studies on the effects of callus induction and differentiation in Gossypium barbadense[J]. Molecular Plant Breeding, 2008, 6(3): 597-602.
[17]  周小凤,张碧瑶,刘冠泽,等.新疆棉花高体细胞胚胎发生能力基因型的筛选[J].分子植物育种,2007,5(6):819-826. ZHOU Xiao-feng, Zhang Bi-yao, Liu Guan-ze, et al. Genotype selection of Xinjiang cottons with higher ability of somatic embryogenesis and plant regeneration[J]. Molecular Plant Breeding, 2007, 5(6): 819-826.
[18]  陈天子,吴慎杰,李飞飞,等.新疆棉花4个主栽品种的体细胞胚胎发生及植株再生[J].作物学报,2008,34(8): 1374- 1380. CHEN Tian-zi, Wu Shen-jie, Li Fei-fei, et al. In vitro regeneration of four commercial cotton(Gossypium hirsutum L.) cultivars grown in Xinjiang, China[J]. Acta Agronomica Sinica, 2008, 34(8): 1374-1380.
[19]  JIN S X, Zhang X L, Nie Y C, et al. Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton[J]. Biologia Plantarum, 2006, 50 (4): 519-524.
[20]  BASSUNER B M, Lam R, Lukowitz W, et al. Auxin and root initiation in somatic embryos of Arabidopsis[J]. Plant Cell Reports, 2007, 26(1): 1-11. [21] QUIROZ F R, Cerda F J, Herrera R, et al. Histological studies on the developmental stages and differentiation of two different SE systems of Coffea Arabica[J]. Plant Cell Reports, 2002, 20:1141-1149.
[21]  SALAJ J, Petrovska B, Obert B, et al. Histological study of embryo-like structures initiated from hypocotyl segments of flax (Linum usitatissimum L.)[J]. Plant Cell Reports, 2005, 24: 590-595.
[22]  KURCZYJSKA E U, Gaj M D, Ujcza A, et al. Histological analysis of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh[J]. Planta, 2007, 226: 619-628.

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