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棉花学报  2012 

低温对转Bt基因棉杀虫蛋白表达及其氮代谢的影响

DOI: 1002-7807(2012)02-0153-06, PP. 153-158

Keywords: Bt棉,低温,杀虫蛋白,氮代谢

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Abstract:

以2个转Bt基因抗虫棉,泗抗1号(常规种)、泗抗3号(杂交种)为材料,研究了低温对转Bt基因抗虫棉叶片中杀虫蛋白表达量和氮代谢的影响。2007年,于盛蕾期、盛花期、盛铃期,将盆栽棉花18℃低温下处理24h。2008年,在18℃低温下处理48h。研究结果表明,低温胁迫显著降低转Bt基因抗虫棉品种叶片中杀虫蛋白表达量。但盛铃期,下降幅度最大。其中低温胁迫24h后,泗抗1号下降23.7%,泗抗3号下降28.1%;低温胁迫48h后,2个品种分别下降52.9%和47.6%。叶片中丙酮酸转氨酶(GPT)活性、游离氨基酸、可溶性蛋白含量与蛋白酶活性在低温胁迫下表现相同趋势。以上结果表明,低温可能导致叶片可溶性蛋白的合成量降低,从而导致杀虫蛋白表达水平的下降。但对可溶性蛋白的分解并未有明显影响。

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