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棉花学报  2012 

棉花杂交种SSR核心引物的筛选与评价

DOI: 1002-7807(2012)03-0207-08, PP. 207-214

Keywords: 棉花,杂交种,SSR标记,核心引物

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Abstract:

为筛选到一套针对棉花杂交种真实性和纯度进行鉴定的核心引物,本实验以79个棉花杂交种为材料,采用分子标记技术,对已公布的2300对SSR引物通过PCR扩增,经初筛和复选,最终获得扩增条带清晰、重复性好且分辨能力强的56对核心引物。利用这56对SSR引物对生产上推广的37个棉花杂交种进行多态性验证,共检测到95个多态性位点,变幅为1~3个;161个基因型,变幅为2~3个。随机抽出10对引物,通过组合方式可以将37个品种完全区分开。对37个品种的SSR分子标记结果进行UPGMA聚类,在相似系数为0.54时,20个长江流域杂交种中有19个被划分到第一类,17个黄河流域品种中有11个被划分到第二类,较好地反映出不同棉区因育种目标的不同所产生的差异。表明该套引物有很好的代表性、实用性和有效性。

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