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棉花学报  2013 

F1代法检测棉铃虫种群对Bt棉的抗性等位基因频率变化

DOI: 1002-7807(2013)03-0240-07, PP. 240-246

Keywords: 转Bt棉,棉铃虫,F1代法,抗性基因频率,抗性检测

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Abstract:

在具有室内高抗Bt棉棉铃虫品系的前提下,F1代法是检测棉铃虫对Bt棉抗性等位基因频率的最简捷、快速的生物测定方法。2010-2012年采用F1代法检测了河北省邱县棉铃虫田间种群对Bt棉的抗性等位基因频率变化。结果表明,2010-2012年采集的122、141及124头田间雄虫中,分别有32、22及43头携带抗性基因,抗性等位基因频率分别为0.131(95%置信限CI0.101~0.162)、0.078(95%CI0.034~0.122)及0.199(95%CI0.124~0.274)。2012年采用带毒饲料法测定河北邱县等4个地区Bt棉田间棉铃虫对Cry1Ac的抗性。结果表明,河北邱县棉铃虫种群的抗性最高(抗性倍数RR为19.2倍),明显高于湖北荆州、湖北枣阳和安徽萧县种群的抗性(RR为4.9~9.3倍)。该地区长期大面积种植转单价Bt棉导致田间棉铃虫抗性进化,需要尽快采取有效的抗性治理措施。

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