全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
棉花学报  2013 

AFLP标记与棉花重要农艺性状的关联研究

DOI: 1002-7807(2013)03-0211-06, PP. 211-216

Keywords: 棉花,AFLP,关联,农艺性状

Full-Text   Cite this paper   Add to My Lib

Abstract:

以陆地棉(GossypiumhirsutumL.)品种中棉所8号和海岛棉(GossypiumbarbadenseL.)品种Pima90-53杂交产生的包含91个单株的BC1F2群体及其衍生BC1F23群体为材料,利用逐步多元回归分析确定分子标记与重要农艺性状的相关关系,为分子标记辅助选择提供依据。试验材料分别种植在保定市郊和沧州青县两个点,每个株行考察衣分、子指、铃重、皮棉重、子棉重5个产量性状和2.5%纤维跨距长度、马克隆值、纤维整齐度、纤维伸长率、纤维比强度5个品质性状。以20对AFLP引物组合产生的125个位点对10个重要农艺性状进行多元线性回归分析,发现其中4对引物组合产生的15个位点与10个性状有着显著相关性(P≤0.05,P≤0.01),而后通过逐步多元回归分析获得6个位点,对9个农艺性状所解释的表型变异为6.2%~30%。结果表明,与9个农艺性状显著相关的6个AFLP位点可以用于未来的分子标记辅助育种计划。

References

[1]  BRUBAKER C L,Paterson A H,Wendel J F.Comparative genetic mapping of allotetraploid cotton and its diploid progenitors[J].Genome,1999,42(4): 184-203.
[2]  PILLAY A A,Myers G O.Genetic diversity in cotton assessed by variation in ribosomal RNA genes and AFLP markers[J].Crop Science,1999,39(6): 1881-1886.
[3]  IQBAL M J,Reddy OUK,Ez-Zak K M,et al.A genetic bottleneck in the evolution under domestication of upland cotton. (Gossypium hirsutum L.) examined using fingerprinting[J].Theoretical and Applied Genetics,2001,103(4): 547-554.
[4]  BRUBAKER C L,Wendel J F.RFLP diversity in cotton[M]// Genetic improvement of cotton,emerging technologies. Melbourne,Australia: Science Publisher Inc,2001: 81-101.
[5]  ADAWY S S,Diab A A,Atia M A M,et al.Construction of genetic linkage map showing chromosomal regions associated with some agronomic traits in cotton[J].Journal of Applied Sciences Research,2008,4: 433-450.
[6]  PREETHA S,Raveendren T S. Molecular marker technology in cotton biotechnology and molecular[J]. Biology Review,2008, 3(2): 32-45.
[7]  CAHILL D J,Schmidt D H.Use of marker assisted selection in a product development breeding program[C/CD]// Gosford,Australia: The Reginal Institute Ltd,2004.
[8]  LIN J J,Kuo J,Ma J,et al.Identification of molecular markers in soybean comparing RFLP,RAPD and AFLP DNA mapping techniques[J].Plant Molecular Biology Reporter,1996,14(2): 156-169.
[9]  VOS P,Hogers R,Bleeker M,et al.AFLP: a new technique for DNA fingerprinting[J].Nucleic Acids Research,1995,23(21): 4407-4414.
[10]  ZHANG Hong-bin,Li Ya-ning,Wang Bao-hua,et al.Recent advances in cotton genomics[J].International Journal of Plant Genomics,2008(2008): 1-20.
[11]  ALTAF M K,Stewart J M,Wajahatullah M K,et al.Molecular and morphological genetics of a trispecies F2 population of cotton[C]// Dugger P,Richter D A. Proc Beltwide Cotton Conf.Memphis,TN: National Cotton Council of America,1997: 448- 452.
[12]  KOHEL R J,Yu J,Park Y H,et al.Molecular mapping and characterization of traits controlling fiber quality in cotton[J].Euphytica,2001,121: 163-172.
[13]  PATERSON A H,Saranga Y,Menz M,et al.QTL analysis of genotype environment interations affecting fiber quality[J].Theoretical and Applied Genetics,2003,106(3): 384-396.
[14]  MEI M,Syed N H,Gao W,et al.Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium)[J].Theoretical and Applied Genetics,2004,108(2): 280-291.
[15]  RONG J K,Abbey C,Bowers J E,et al.A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization,transmission and evolution of cotton (Gossypium)[J].Genetics,2004,166(1): 389-417.
[16]  SARANGA Y,Menz M,Jiang C,et al.Genetic and physiological dissection of adaptations associated with cotton productivity under arid conditions[J].Plant,Cell and Environment,2004, 27(3): 263-277.
[17]  JAMES E,Frelichowski J,Michael B,et al.Cotton genome mapping with new microsatellites from Acala 'Maxxa' BAC-ends[J].Molecular Genetic and Genomics,2006,275(5): 479-491.
[18]  LACAPE J M,Nguyen T B,Courtois B,et al.QTL analysis of cotton fiber quality using multiple Gossypium hirsutum × Gossypium barbadense backcross generations[J].Crop Science,2005,45(1): 123-140.
[19]  WU Ji-xiang,Jenkins J N,McCarty J C,et al.AFLP marker associations with agronomic and fiber traits in cotton[J].Euphytica,2007,153(1/2): 153-163.
[20]  PATERSON A H,Brubaker C L,Wendel J F.A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis[J]. Plant Molecular Biology Reporter,1993,11(2): 122-127.
[21]  赵 宏,王省芬,张桂寅,等.棉花AFLP 技术体系的摸索与建立[J].河北农业大学学报,2002,25(1):1-4. ZHAO Hong,Wang Xing-fen,Zhang Gui-yin,et al.The study and establishment of AFLP technical system in cotton[J].Journal of Agricultural University of Hebei,2002,25(1): 1-4.
[22]  杨鑫雷,王志伟,张桂寅,等.棉花分子遗传图谱构建和纤维品质性状QTL分析[J].作物学报,2009,35(12):2159- 2166. YANG Xin-lei,Wang Zhi-wei,Zhang Gui-yin,et al.Construction of molecular genetic map and QTL analysis of fiber quality in cotton[J].Acta Agronomica Sinica,2009,35(12): 2159- 2166.
[23]  KRAAKMAN A T W,Niks R E,Van Den berg P M M M,et al.Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars[J].Genetics,2004,168(1): 435-446.
[24]  徐云碧,朱立煌.分子数量遗传学[M].北京:中国农业出版社,1994. XU Yun-bi,Zhu Li-huang.Molecular quantitative genetics[M].Beijing: China Agricultural Press,1994.
[25]  RONG J K,Feltus F A,Waghmare V N,et al.Meta-analysis of polyploid cotton QTL shows unequal contributions of subgenomes to a complex network of genes and gene clusters implicated in lint fiber development[J].Genetics,2007,176(4): 2577-2588.
[26]  ZHANG Tian-zhen,Yuan You-lu,Yu J,et al.Molecular tagging of major QTL for fiber strength in upland cotton and its marker-assisted selection[J].Theoretical and Applied Genetics,2003, 106(2): 262-268.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133