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棉花学报  2013 

农杆菌介导的基因敲除技术在丝状真菌基因功能研究中的应用

DOI: 1002-7807(2013)03-0262-07, PP. 262-268

Keywords: 农杆菌介导的遗传转化,基因敲除,同源重组,丝状真菌,功能研究

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Abstract:

近年来不少丝状真菌全基因组测序已经完成或正在进行,基因功能研究已成为真菌研究的一个热点。利用反向遗传学的方法敲除基因是研究丝状真菌功能基因的常用方法之一,基因敲除是通过外源DNA与染色体DNA之间的同源重组,可以敲除整个基因,或者将一个基因替换成该基因的等位基因,或者用一个调控启动子替换一个基因正常的启动子,从而精细地定点修饰和改造基因DNA片段的技术。本文简述了利用农杆菌介导的遗传转化方法(ATMT)进行基因敲除的技术在丝状真菌基因组功能研究中的应用。

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