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棉花学报  2013 

棉花转录因子GhWRKY4基因的克隆及特征分析

DOI: 1002-7807(2013)03-0205-06, PP. 205-210

Keywords: GhWRKY4基因,棉花,转录因子,胁迫

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Abstract:

本研究从陆地棉中克隆得到GhWRKY4基因,全长cDNA是1281bp,包含1083bp的开放阅读框,编码的360个氨基酸属于IIcWRKY转录因子家族。分析GhWRKY4基因结构表明其有三个外显子和两个内含子。GhWRKY4蛋白的亚细胞定位实验表明其定位在洋葱表皮细胞核。实时荧光定量分析结果显示GhWRKY4在棉花的根、茎、叶和花中均有表达。染色体步移得到了GhWRKY4的5’端侧翼序列,生物信息学软件预测表明GhWRKY4包含一系列和胁迫基因调控相关的反式作用元件,表明GhWRKY4参与植物对盐害和冷害胁迫的反应。

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