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棉花学报  2013 

棉花黄萎病拮抗细菌Z-5菌株的定殖能力检测

DOI: 1002-7807（2013）06-0510-07, PP. 510-516

Keywords: 棉花黄萎病,拮抗,定殖,抗生素标记

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Abstract:

拮抗微生物能否在植株根围成功定殖是植物病害生物防治的第一步。以棉田土壤中分离的对棉花黄萎病有较高生物防治效果的芽孢杆菌Z-5菌株为材料，采用抗利福平和抗病原真菌法标记Z-5菌株，筛选出在300μg·mL-1利福平的NA培养基上稳定生长，对棉花黄萎病病原真菌有拮抗作用、菌落形态和菌体形态均未改变的Z-5突变体菌株。利用标记的Z-5菌株对棉花种子进行浸种、拌种和浇土处理，在含有利福平的平板上通过稀释涂布法定期检测棉花植株根际土壤、根、茎、叶中Z-5菌株的数量。结果表明，浇土组棉花根际土壤拮抗细菌Z-5平均数量为49.72×104cfu·g-1，浸种组为0.49×104cfu·g-1，拌种组为2.07×104cfu·g-1。浇土组根内Z-5平均数量为4.93×104cfu·g-1，浸种组为4.12×104cfu·g-1，拌种组为3.29×104cfu·g-1。浇土组茎内Z-5平均数量为1.32×104cfu·g-1，浸种组为0.17×104cfu·g-1，拌种组为0.74×104cfu·g-1。浇土组叶片Z-5平均数量为0.27×104cfu·g-1，浸种组为0.17×104cfu·g-1，拌种组为0.22×104cfu·g-1。表明Z-5菌株能够在棉花根际土壤及植株体内有效定殖；采用浇土法处理棉花种子，Z-5菌株在棉花植株体内及根际土壤定殖效果最好，优于浸种法和拌种法。

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