全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
棉花学报  2014 

棉蚜保幼激素环氧水解酶基因克隆及表达初步分析

DOI: 1002-7807(2014)04-0326-08, PP. 326-333

Keywords: 棉蚜,保幼激素环氧水解酶,基因克隆,表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

保幼激素环氧水解酶(Juvenilehormoneepoxidehydrolase,JHEH)在保幼激素(Juvenilehormone,JH)的代谢途径中起重要的降解作用。为初步分析JHEH在棉蚜(AphisgossypiiGlover)生长发育中可能存在的功能,本研究通过转录组测序并结合RACE技术,克隆AgosJHEH基因,其开放阅读框为1401bp,编码466个氨基酸,预测蛋白质分子量为52.6kDa,等电点8.19。N末端存在由20个氨基酸组成的疏水性信号肽序列,存在催化三联体Asp(230)、Glu(408)、His(437)氨基酸残基以及阴氧离子洞Tyr(304)、Tyr(378)和HGWP花样结构氨基酸残基,与豌豆蚜氨基酸序列有很高的同源性。RT-qPCR结果表明AgosJHEH在棉蚜无翅若虫3、4龄表达量显著低于其他龄期,在无翅成虫中各生命时期没有显著差异,有翅成虫腹部显著低于翅中的表达量。本研究首次对AgosJHEH进行结构分析及功能预测,为深入理解其对棉蚜JH滴度的调控机制及开发新型杀虫剂奠定基础。

References

[1]  刘建涛, 赵利, 苏伟. 昆虫保幼激素及其类似物的应用研究进展[J]. 安徽农业科学, 2006, 34(11): 2446-2448.
[2]  Liu Jiantao, Zhao Li, Su Wei. Research progress in the juvenile hormone application and its analogs[J]. Journal of Anhui Agricultural Sciences, 2006, 34(11): 2446-2448.
[3]  Wyatt G R, Davey K G. Cellular and molecular actions of juvenile hormone. II. roles of juvenile hormone in adult insects[J].Advances in Insect Physiology, 1996, 26: 1-156.
[4]  欧阳迎春, 李胜. 保幼激素及其代谢产物的HPLC分离方法的改进和应用[J]. 昆虫学报, 2003, 46(3): 282-287.
[5]  Ouyang Yingchun, Li Sheng. Modification and application of a high performance liquid chromatography method to separate juvenile hormones and their metabolites[J]. Acta Entomologica Sinica, 2003, 46(3): 282-287.
[6]  Gilbert L I, Granger N A, Roe R M. The juvenile hormones: historical facts and speculations on future research directions[J]. Insect Biochemistry and Molecular Biology, 2000, 30: 617-644.
[7]  Hammock B D. Regulation of juvenile hormone titer: degradation[M] // Kerkut G A, Gilbert L I. Comprehensive insect physiology, biochemistry, and pharmacology. Oxford, UK: Pergamon Press, 1985: 431-472.
[8]  Kamita S G, Hinton A C, Wheelock C E, et al. Juvenile hormone (JH) esterase: why are you so JH specific?[J]. Insect Biochemistry and Molecular Biology, 2003, 33: 1261-1273.
[9]  Kallapur V L, Majumder C, Roe R M. In vivo and in vitro-tissue specific metabolism of juvenile hormone during the last stadium of the cabbage looper, Trichoplusia ni[J]. Journal of Insect Physiology, 1996, 42: 181-190.
[10]  Halarnkar P P, Jackson G P, Straub K M, et al. Juvenile hormone catabolism in Manduca sexta: homologue selectivity of catabolism and identification of a diol-phosphate conjugate as a major end product[J]. Experientia, 1993, 49: 988-994.
[11]  Christine B, Muller, Iain S, et al. The role of nutrition, crowding, and interspecfic interactions in the development of winged aphids [J]. Ecological Entomology, 2001, 26: 330-340.
[12]  Pfaffl M W. A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Research, 2001, 29(900): 2002-2007.
[13]  Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-??CT Method[J]. Methods, 2001, 25: 402-408.
[14]  Zhang Qirui, Xu Weihua, Chen Fusheng, et al. Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori[J]. Insect Biochemistry and Molecular Biology, 2005, 35: 153-164.
[15]  Touhara K, Prestwich G D. Juvenile hormone epoxide hydrolase: photoaffinity labeling, purification, and characterization from tobacco hornworm eggs[J]. The Journal of Biological Chemistry, 1993, 268: 19604-19609.
[16]  Wojtasek H, Prestwich G D. An insect juvenile hormone-specific epoxide hydrolase is related to vertebrate microsomal epoxide hydrolases[J]. Biochemical and Biophysical Research Communications, 1996, 220: 323-329.
[17]  Atsushi S, Ogura T, Tsubota T, et al. characterization of juvenile hormone epoxide hydrolase and related genes in the larval development of silkworm Bombyx mori[J]. Bioscience, Biotechnology, and Biochemistry, 2010, 74(7):1421-1429.
[18]  宁长明, 吴孔明, 高希武, 等.棉铃虫保幼激素环氧水解酶基因的克隆及其原核表达[J]. 环境昆虫学报, 2009, 31(2): 114- 123.
[19]  Ning Changming, Wu Kongming, Gao Xiwu, et al. Cloning of cDNA-encoding juvenile hormone epoxide hydrolase in Helicoverpa armigera (Hübner) and its expression in E.coli[J]. Journal of Environmental Entomology, 2009, 31(2): 114-123.
[20]  李晓静. 小菜蛾保幼激素环氧水解酶基因的克隆及其与抗性适合度变化的联系[D]. 福州: 福建农林大学, 2012.
[21]  Li Xiaojing. The fitness cost of the insecticide-resistant diamondback moth and its association with the JHEH gene[D]. Fuzhou: Fujian Agriculture and Forestry University, 2012.
[22]  Mackert A, Hartfelder K, Bitondi M M G, et al. The juvenile hormone (JH) epoxide hydrolase gene in the honey bee (Apismelli fera) genome encodes a protein which has negligible participation in JH degradation[J]. Journal of Insect Physiology, 2010, 56: 1139-1146.
[23]  Tsubota T, Nakakura T, Shiotsuki T. Molecular characterization and enzymatic analysis of juvenile hormone epoxide hydrolase genes in the red flour beetle Tribolium castaneum[J]. Insect Molecular Biology, 2010, 19(3): 399-408.
[24]  Keiser K C L, Brandt K S, Silver G M, et al. Cloning, partial purification and in vivo developmental profile of expression of the juvenile hormone epoxide hydrolase of Ctenocephalides felis[J]. Archives of Insect Biochemistry and Physiology, 2002, 50: 191-206.
[25]  Taniai K, Inceoglu A B, Yukuhiro K, et al. Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster[J]. European Journal of Biochemistry, 2003, 270: 4696-4705.
[26]  Harris S V, Thompson D M, Linderman R J, et al. Cloning and expression of a novel juvenile hormone-metabolizing epoxide hydrolase during larval-pupal metamorphosis of the cabbage looper, Trichoplusia ni[J]. Insect Molecular Biology, 1999, 8(1): 85-96.
[27]  朱道弘. 昆虫翅型分化的调控及翅多型性的进化[J].昆虫知识, 2009, 46(1): 11-16.
[28]  Zhu Daohong. Regulatory mechanism and evolution of insect wing polymorphism[J]. Chinese Bulletin of Entomology, 2009, 46(1): 11-16.
[29]  Roff D A. The evolution of wing dimorphism in insects[J]. Evolution, 1986, 40(5): 1009-1020.
[30]  Douglas D A, Roe R M. Regulation of JH epoxide hydrolase versus JH esterase activity in the cabbage looper, Trichoplusia ni, by juvenile hormone and xenobiotics[J]. Journal of Insect Physiology, 2005, 51: 523-535.
[31]  Khalil S M S, Anspaugh D D, Roe RM. Role of juvenile hormone esterase and epoxide hydrolase in reproduction of the cotton bollworm, Helicoverpa zea[J]. Journal of Insect Physiology, 2006, 52: 669-678.
[32]  夏耀民, 孙名帅. 11种杀虫剂对棉蚜的田间防效研究[J]. 现代农业科技, 2013(17): 155-156.
[33]  Xia Yaomin, Sun Mingshuai. Study on field trials of 11 insecticides for the control of Aphis gossypii Glover[J]. Modern Agricultural Science and Technology, 2013(17): 155-156.
[34]  Severson T F, Goodrow M H, Morisseau C, et al. Urea and amide-based inhibitors of the juvenile hormone epoxide hydrolase of the tobacco hornworm(Manduca sexta: Sphingidae)[J]. Insect Biochemistry and Molecular Biology, 2002, 32: 1741- 1756.
[35]  Lindernman R J, Roe R M, Harris S V, et al. Inhibition of insect juvenile hormone epoxide hydrolase: asymmetric synthesis and assay of glycidol-ester and epoxy-ester inhibitors of Trichoplusia ni epoxide hydrolase[J]. Insect Biochemistry and Molecular Biology, 2000, 30: 767-774.
[36]  薛丽, 肖达, 卢延, 等. 转基因棉对棉蚜生长发育及其主要代谢物质含量的影响[J]. 华北农学报, 2011, 26(4): 115-120.
[37]  Xue Li, Xiao Da, Lu Yan, et al. Effects of transgenic insect-resistant cotton on development and metabolite content of cotton aphid Aphis gossypii[J]. Acta Agriculturae Boreali-Sinica, 2011, 26(4): 115-120.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133