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棉花学报  2014 

绿色棉胚珠离体培养体系优化及光照对纤维生长发育的影响

DOI: 1002-7807(2014)04-0318-08, PP. 318-325

Keywords: 绿色棉,胚珠,离体培养,纤维生长

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Abstract:

在优化绿色棉离体培养体系的基础上,以绿色棉品种绿絮棉1号、白色棉品种泗棉三号(对照)为材料,比较了光培养和暗培养对鲜胚珠质量、纤维长度、纤维素含量等生理生化指标影响,来探讨光照对绿色棉纤维生长发育的影响。结果表明花后2d的绿色棉胚珠较适宜离体培养,其适宜的消毒方式为75%的乙醇10min+0.1%升汞10min;离体培养的适宜激素及其浓度为GA31.0μmol·L-1+IAA5.0μmol·L-1+ZR2.5μmol·L-1,3种激素对胚珠鲜质量和纤维伸长影响大小的顺序为ZR>IAA>GA3。光培养能够抑制纤维的伸长、β-1,3-葡聚糖酶活性和培养前期的纤维蔗糖合成酶活性;但能够促进胚珠的生长、纤维素的合成、纤维PAL活性和培养后期的纤维蔗糖合成酶活性,提高黄酮含量。可见,光照能够对绿色棉纤维生长发育产生一定的影响。

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