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棉花学报  2014 

陆地棉转录因子GhNAC78基因的特征及功能分析

, PP. 283-289

Keywords: GhNAC78基因,陆地棉,转录因子,转录激活域,叶片衰老

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Abstract:

本实验室从陆地棉中克隆得到GhNAC78,分析了其基因特征及功能。该基因cDNA全长为937bp,开放阅读框为876bp,编码291个氨基酸,隶属于NAM转录因子亚家族。体外转录激活实验表明,GhNAC78具有转录激活活性。上游启动子区1537bp序列的生物信息学分析可知其包含多种激素、胁迫和光响应元件,表明GhNAC78广泛地参与植物生长发育和胁迫响应的调控。荧光定量结果显示,GhNAC78在棉花叶片衰老过程中高调表达,推测其参与叶片衰老的调控。

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