全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
棉花学报  2014 

低温和湿度胁迫对盛铃期Bt棉叶片Bt蛋白表达量的影响

, PP. 290-294

Keywords: Bt棉,Bt蛋白,低温,相对湿度

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用盆栽试验,以转Bt基因的常规品种DP410B和泗抗1号、杂交种岱杂1号和泗抗3号为材料,探讨盛铃期18℃低温下不同湿度对Bt棉叶片Bt蛋白表达量的影响。结果表明,2010年,低温下90%、70%、50%这3种湿度胁迫6h后,DP410B和岱杂1号叶片Bt蛋白含量比各自对照(温度为常温,湿度70%)显著降低,但泗抗1号和泗抗3号没有显著变化;2011年,当低温下90%、50%湿度胁迫时间延长至48h后,4个品种叶片中Bt蛋白含量比各自对照均显著下降。其中岱杂1号和DP410B在胁迫12h后便开始显著低于各自对照,而泗抗1号和泗抗3号则在24h以后才显著低于对照。说明随低温和湿度胁迫时间延长,Bt棉叶片中Bt蛋白含量下降效应逐渐增大,但不同品种间存在一定差异。

References

[1]  郭香墨, 范术丽, 王红梅, 等. 我国棉花育种技术的创新与成就[J]. 棉花学报, 2007, 19(5): 323-330.
[2]  Guo Xiangmo, Fan Shuli, Wang Hongmei, et al. Achievements of technical innovation about cotton genetics and breeding in China[J]. Cotton Science, 2007, 19(5): 323-330.
[3]  夏敬源, 邹奎, 马志强, 等. 国产转基因抗虫棉技术集成创新与推广应用[J]. 中国棉花, 2006, 33(10): 2-5.
[4]  Xia Jingyuan, Zou Kui, Ma Zhiqiang, et al. Integrated innovation and promotion of domestic transgenic cotton technology[J]. Chinese Cotton, 2006, 33(10): 2-5.
[5]  崔洪志, 郭三堆. 我国抗虫转Bt基因棉花研究取得重大进展[J].中国农业科学, 1996, 29(1): 93.
[6]  Cui Hongzhi, Guo Sandui. Important progress on study of insect-resistance of transgeneic cotton in China[J]. Scientia Agricultura Sinica,1996, 29(1): 93.
[7]  林毅, 郑后今, 高用明, 等. 抗虫棉抗虫特性及产量性状的研究[J].安徽农业大学学报: 自然科学版, 1998, 25(2): 174-177.
[8]  Lin Yi, Zheng Houjin, Gao Yongming, et al. Study on the anti-bollworm characteristics and yield traits of anti-bollworm cotton[J]. Journal of Anhui Agricultural University: Natural Science Edition, 1998, 25(2): 174-177.
[9]  Dong Hezhong, Li Weijiang. Variability of endotoxin expression in Bt transgenic cotton[J]. Journal of Agronomy and Crop Science, 2007, 193: 21-29.
[10]  赵奎军, 赵建周, 范贤琳, 等. 我国转Bt基因抗虫棉对棉铃虫抗虫性时空动态的研究[J]. 沈阳农业大学学报, 1999, 30(3) :389.
[11]  Zhao Kuijun, Zhao Jianzhou, Fan Xianlin, et al. Study on temporal and spatial dynamics of the resistance of Chinese transgenic cotton cultivars to bollworm[J]. Journal of Shenyang Agricultural University, 1999, 30(3): 389 .
[12]  Kaiser J. Pest overwhelm Bt cotton crop[J]. Science, 1996, 273: 423.
[13]  Jiang Linjian, Duan Liusheng, Tian Xiaoli, et al. NaCl salinity stress decreased Bacillus thuringiensis(Bt) protein content of transgenic Bt cotton (Gossypium hirsutum L.) seedlings[J]. Environmental and Experimental Botany, 2006, 55: 315-320.
[14]  Luo Zhen, Dong Hezong, Li Weijiang, et al. Individual and combined effects of salinity and waterlogging on Cry1Ac expression and insecticidal efficacy of Bt cotton[J]. Crop Protection, 2008, 27: 1485-1490.
[15]  张祥, 刘晓飞, 吕春花, 等. 低温对转Bt基因棉杀虫蛋白表达及氮代谢影响[J].棉花学报, 2012, 24(2): 153-158.
[16]  Zhang Xiang, Liu Xiaofei, Lyu Chunhua, et al. Effect of low temperature on the insecticidal properties and nitrogen metabo- lism of Bt cotton[J]. Cotton Science, 2012, 24(2): 153-158.
[17]  陈源, 顾超, 王桂霞, 等. 蕾期低温及湿度胁迫对Bt棉杀虫蛋白表达量的影响[J]. 作物学报, 2013, 39(1): 184-189.
[18]  Chen Yuan, Gu Chao, Wang Guixia, et al. Effect of stress of 18℃ and different relative humidity on Bt protein expression at squaring stage in Bt cotton[J]. Acta Agronomic Sinca, 2013, 39(1): 184-189.
[19]  陈松, 吴敬音, 何小兰, 等. 转基因抗虫棉组织中Bt毒蛋白表达量的ELISA测定[J]. 江苏农业学报, 1997, 13(3): 154-156.
[20]  Chen Song, Wu Jingyin, He Xiaolan, et al. Quantification using ELISA of Bacillus thuringiensis insecticidal protein expressed in the tissue of transgenic insect resistant cotton[J]. Jiangsu Journal of Agricultural Science, 1997, 13(3): 154-156.
[21]  张旭晖, 黄毓华. 1999 年江苏省棉花减产气象条件分析[J]. 中国棉花, 2000, 27(9): 15-17.
[22]  Zhang Xuhui, Huang Yuhua. The climate analysis of Jiangsu province for cotton yield decrease in 1999[J]. China Cotton, 2000, 27(9): 15-17.
[23]  卞海云, 陈兵林, 周治国, 等. 低温条件下外源生理活性物质对棉铃发育的影响[J]. 西北植物学报, 2005, 25(9): 1785-1790.
[24]  Bian Haiyun, Chen Binglin, Zhou Zhiguo, et al. Effect of exogenous and physiologically activity substances on the development of cotton bolls at low temperature[J]. Acta Bot Boreal-Occident Sin, 2005, 25(9): 1785-1790.
[25]  Hodges H F, Reddy K R, McKinion J M. Temperature effects on cotton[J]. Mississippi Agric For Exp Stn Bull, 1993, 990: 1-15.
[26]  Reddy K R, Reddy V R, Hodges H F. Temperature effects on early season cotton growth and development[J]. Agronomy Journal, 1992, 84: 237-243.
[27]  Reddy V R, Reddy K R, Baker D N. Temperature effects on growth and development of cotton during the fruiting period[J]. Agron J, 1991, 83: 211-217.
[28]  Adamczyk J J, Meredith W R. Genetic basis for the variability of Cry1Ac expression among commercial transgenic Bacillus thuringiensis (Bt) cotton cultivars in the United States[J]. Journal of Cotton Science, 2004, 8: 17-23.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133