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棉花学报  2012 

海岛棉两种转Bt基因方法的研究

DOI: 1002-7807(2012)05-0393-06, PP. 393-398

Keywords: 海岛棉,农杆菌菌液喷雾法,质粒注射法,Bt基因

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Abstract:

以新疆海岛棉军海1号为受体,利用农杆菌菌液喷雾法(Floralspray)和质粒注射法(Plasmidinjection)导入外源Bt基因。经过对转化植株T1和T2代大田筛选和PCR、Southern检测,并经孟德尔遗传规律符合度分析,结果表示农杆菌菌液喷雾法T0代成铃率比质粒注射法高8.3百分点;两种方法均可获得转基因植株,农杆菌菌液喷雾法较质粒注射法转化率高3.4百分点;农杆菌菌液喷雾处理后代较符合孟德尔遗传规律且单拷贝插入几率高。因此,农杆菌菌液喷雾法优于质粒注射法。

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