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棉花学报  2012 

陆地棉苗期低温胁迫下cDNA-AFLP差异表达分析

DOI: 1002-7807(2012)05-0386-07, PP. 386-392

Keywords: 陆地棉,低温胁迫,cDNA-AFLP,基因差异表达,转录衍生片段

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Abstract:

以陆地棉品系棕彩选1号为材料,利用cDNA-AFLP技术研究棉花低温处理前后基因表达的差异。试验采用64对引物组合进行扩增,共获得2321个条带,从中得到34条表达存在差异的条带。根据表达模式的不同,对其中表达存在差异的22个转录衍生片段(Transcriptsderivedfragments,TDFs)进行了克隆、测序和序列分析。结果表明在低温胁迫条件下获得的18条TDFs中,上调表达2条(11%),下调表达9条(50%),另外7条(39%)为瞬时表达;而在恢复2d后得到了4条特异表达的TDFs。22个TDFs中,21个TDFs与NCBI中已知功能基因存在同源性,这些基因包括假定蛋白、转座子、线粒体、过氧化物酶体增殖物激活受体和逆境胁迫反应关键基因等。另有1个TDFs无同源序列。

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