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棉花学报  2013 

应用纳米磁珠荧光PCR检测棉花曲叶病毒

DOI: 1002-7807(2013)01-0090-05, PP. 90-94

Keywords: 棉花曲叶病毒,实时荧光PCR,检测

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Abstract:

为了提供快速灵敏检测棉花曲叶病毒(Cottonleafcurlvirus,CLCuV)的技术以防止其传播扩散,本研究根据CLCuV外壳蛋白基因(Coatprotein,CP)保守序列设计了引物和TaqMan探针,并结合纳米磁珠(Magneticnanoparticles,MNPs)建立了该病毒的MNP实时荧光PCR(Real-timePCR)检测方法。该方法检测阈值约为525fg·μL-1DNA。通过对CLCuV、非洲木薯花叶病毒、烟草线条病毒、黄瓜花叶病毒及番茄斑萎病毒的检测表明,该方法具有良好的特异性;同时该方法无需任何PCR后处理,交叉污染风险小。本方法可用于CLCuV的快速检测。

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