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棉花学报  2013 

利用重组自交系定位棉花第22染色体短臂的纤维品质性状的QTL

DOI: 1002-7807(2013)01-0024-06, PP. 24-29

Keywords: 重组自交系,置换系,遗传连锁图谱,纤维品质性状,QTL

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Abstract:

以TM-1的染色体片段代换系CSB22sh和TM-1杂交,构建了包含104个家系的重组自交系(RILs)群体。利用74对具有多态性位点的引物进行检测,构建了包含61个多态性位点,长度为76.93cM的遗传图谱,平均标记间距离1.26cM。利用此遗传图谱结合重组自交系群体4个环境下的5个纤维品质性状进行QTL定位,共定位出12个QTLs,解释性状表型变异的2.45%~21.11%;在1,2,3,4个环境中同时检测到的QTLs分别有9,1,1,1个。而利用4个环境平均值进行联合分析定位出个4个QTLs,纤维长度和纤维整齐度的QTLs均为2个,解释性状表型变异的14.37%~19.97%,并且纤维长度和整齐度的QTL在相同的位置。多环境下检测到的QTL可能对标记辅助选择有实际应用价值。

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