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棉花学报  2013 

棉花细菌人工染色体文库构建方法探讨

DOI: 1002-7807(2013)01-0009-08, PP. 9-16

Keywords: 细菌人工染色体文库,野生棉,构建方法

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Abstract:

细菌人工染色体(Bacterialartificialchromosome,BAC)文库是开展基因组测序、基因图位克隆、分子标记、物理作图等研究的重要基因组资源。本文在构建了二倍体野生棉阿非利加棉(Gossypiumherbaceumvar.africanum)BAC文库的基础上,就棉花细菌人工染色体基因组文库构建过程中高分子量基因组DNA的提取、部分酶切片段选择、DNA的回收、连接转化以及BAC文库的保存等过程中一些细节和注意事项进行了比较详细的分析比较,希望能为棉花BAC文库的构建提供一些可供借鉴的经验。

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