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棉花学报  2013 

陆地棉水通道蛋白GhNIP6.1基因的克隆及表达分析

DOI: 1002-7807(2013)01-0001-08, PP. 1-8

Keywords: 陆地棉,水通道蛋白,GhNIP6.1,表达序列标签

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Abstract:

根据EST拼接的序列设计引物,利用RT-PCR技术,从陆地棉品种苏棉18中克隆获得了一个水通道蛋白基因GhNIP6.1。该基因开放阅读框包含903个核苷酸,编码300个氨基酸,分子量为30.97kD,理论等电点为8.99。GhNIP6.1蛋白的生物信息学分析表明GhNIP6.1含有6个跨膜区,由5个环相连,其中环A、C和E在细胞膜外,环B、D和蛋白的N、C末端都位于细胞膜内,N末端79个氨基酸,C末端18个氨基酸,具有MIP家族典型的保守氨基酸序列;该蛋白的三级结构同拟南芥的AtNIP6.1非常相似,亚细胞定位也同AtNIP6.1一致,可能位于质膜中。进化分析发现,GhNIP6.1蛋白同拟南芥的AtNIP6.1蛋白相似性最高。进一步扩增棉花核基因组获得了2021bp的DNA序列,它包含5个外显子和4个内含子,所有外显子/内含子交接点都遵从gt/ag剪接规则。半定量分析表明,该基因在根、茎和叶中都有表达,其中真叶含量较高,子叶中没有表达。

References

[1]  TAKANO J,Tanaka M,Toyoda A,et al. Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways[J]. Proceedings of the National Academy of Sciences,2010,107(11): 5220-5225.
[2]  TANAKA M,Wallace I S,Takano J,et al. NIP6;1 is a boric acid channel for preferential transport of boron to growing shoot tissues in Arabidopsis[J]. Plant Cell,2008,20(10): 2860-2875.
[3]  DENKER B M,Smith B L,Kuhaida F P,et al. Identification,purification,and partial characterization of a novel Mr 28 000 integral membrane protein from erythrocytes and renal tubules[J]. Journal of Biological Chemistry,1988,263(30): 15634-15642.
[4]  PRESTON G M,Agre P.Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family[J]. Proceedings of the National Academy of Sciences,1991,88(24): 11110-11114.
[5]  PRESTON G M,Carroll T P,Guggino W B,et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein [J]. Science,1992,17(256): 385-387.
[6]  H?FTE H,Hubbard L,Reizer J,et a1. Vegetative and seed-specific forms of tonoplast intrinsic protein in the vacuolar membrane of Arabidopsis thaliana[J]. Plant Physiology,1992,99(2): 561-570.
[7]  BAIGE I,Schaffner A R,Affenzeller M J,et al. Plant aquaporins [J]. Physiologia Plantarum,2002,115 (2): 175-182.
[8]  CHAUMONT F,Barrieu F,Wojcik E,et al. Aquaporins constitute a large and highly divergent protein family in maize[J]. Plant Physiology,2001,125 (3): 1206-1215.
[9]  QUIGLEY F,Rosenberg J M,Shachar-Hill Y,et a1. From genome to function: the Arabidopsis aquaporins[J]. Genome Biology,2001,3(1): 1-17.
[10]  CHANMONT F,Moshelion M,Daniels M J. Regulation of plant aquaporin activity[J]. Biology Cell,2005,97(10): 749-764.
[11]  TAKANO J,Wada M,Ludewig U,et al. The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation[J]. Plant Cell,2006,18(6): 1498-1509.
[12]  MAUREL C,Verdoucq L,Luu D T,et al. Plant aquaporins: membrane channels with multiple integrated functions[J]. Annual Review of Plant Biology,2008(59): 595-624.
[13]  PARK W,Scheffler B E,Bauer P J,et al. Identification of the family of aquaporin genes and their expression in upland cotton (Gossypium hirsutum L.)[J]. BMC Plant Biology,2010(10): 142-158.
[14]  胡根海,喻树迅.利用改良的CTAB 法提取棉花叶片总RNA[J].棉花学报,2007,19(1):69-70. HU Gen-hai,Yu Shu-xun. Extraction of high-quality total RNA in cotton leaf with improved CTAB method[J]. Cotton Science,2007,19(1): 69-70.
[15]  孙志栋,王学德,倪西源,等.棉花DNA提取方法的探讨[J].浙江农业学报,2004,16(4):177-181. SUN Zhi-dong,Wang Xue-de,Ni Xi-yuan,et al. Primarily study on isolation of genomic DNA from cotton[J]. Acta Agriculturae Zhejiangensis,2004,16(4): 177-181.
[16]  CHEN Jie-yin,Dai Xiao-feng. Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana[J]. Planta,2010,231(4): 861-873.
[17]  FUIIYOSHI Y,Mitsuoka K,de Groot B L,et al. Structure and function of water channels[J]. Current Opinion in Structural Biology,2002,12(4): 509-515.
[18]  CHANMONT F,Moshelion,Daniels M J . Regulation of plant aquaporin activity[J]. Biology Cell,2005,97(10): 749- 764.

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