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棉花学报  2013 

适用于转录组测序的棉花幼根总RNA提取方法筛选

DOI: 1002-7807(2013)04-0372-005, PP. 372-376

Keywords: 棉花,转录组,RNA提取方法,根部

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Abstract:

棉花幼根中含有大量酚类物质、多糖和单宁等多种次生代谢物,且根部取样时会带有少量的泥土和有机质等污染物,因此棉花幼根RNA提取难度较大。本文采用两种试剂盒法和改良的异硫氰酸胍法,筛选适合转录组测序的提取棉花幼根RNA的方法。与试剂盒法相比,改良的异硫氰酸胍法具有浓度高、质量好等特点,能够满足转录组测序的要求。

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