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棉花学报  2013 

棉花航天诱变芽黄突变体蛋白组学分析

DOI: 1002-7807(2013)04-0345-07, PP. 345-351

Keywords: 棉花,芽黄突变体,蛋白质组,质谱分析

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Abstract:

以中棉所58及其航天诱变芽黄突变体中棉所58vsp倒2叶为材料,利用等电聚焦和第二向SDS-PAGE技术获得棉花叶片总蛋白图,通过ImageMaster-2DElite7.0分析软件分析各个差异蛋白在两种叶片中的相对表达量,并进行MALDI-TOF/TOF鉴定。结果表明,从中棉所58及其突变体的双向电泳图谱中共检测到41个差异蛋白点,这些差异蛋白质点的等电点分布集中在4.0~7.0之间,分子量分布集中在15.0~95.0kD之间,进一步质谱分析鉴定后获得了14个差异蛋白点,包括核酮糖-1,5二磷酸羧化酶/加氧酶、S-腺苷甲硫氨酸合成酶、黄烷酮3-羟化酶等多种蛋白,涉及到光合作用和光呼吸、乙烯和多胺的合成、类黄酮的合成等生物代谢途径。

References

[1]  KILLOUGH D T,Horlacher W R.The inheritance of virescent yellow and red plant color in cotton[J].Genetics,1933(18): 329- 334.
[2]  肖松华,张天真,潘家驹.陆地棉芽黄近等基因系研究[J].棉花学报,1996,8(5):235-240. XIAO Song-hua,Zhang Tian-zhen,Pan Jia-ju.Study on virescent near-isogenic line in upland cotton[J].Acta Cossypii Sinica,1996, 8(5): 235-240.
[3]  宋美珍,杨兆光,范术丽,等.一个短季棉芽黄基因型的鉴定及生理生化分析[J].中国农业科学,2011,44(18):3709- 3720. SONG Mei-zhen,Yang Zhao-guang,Fan Shu-li,et al.Physiological and biochemical analysis and identification of a short season cotton virescent mutant[J].Scientia Agricultura Sinica,2011,44(18): 3709-3720.
[4]  WU Zi-ming,Zhang Xin,He Bing,et al.A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis[J].Plant Physiol,2007,145(1): 29- 40.
[5]  HALL L N,Rossini L,Cribb L,et al.GOLDEN 2: a novel transcriptional regulator of cellular differentiation in the maize leaf [J].Plant Cell,1998,10(6): 925-936.
[6]  YASUMURA Y,Moylan E C,Langdale J A.A conserved transcription factor mediates nuclear control of organelle biogenesis in anciently diverged land plants[J].Plant Cell,2005,17(7): 1894-1907.
[7]  ISHIJIMA S,Uchibori A,Takagi H,et al.Light-induced increase in free Mg2+ concentration in spinach chloroplasts: Measurement of free Mg2+ by using a fluorescent probe and necessity of stromal alkalinization[J].Arch Biochem Biophys,2003,412(1): 126-132.
[8]  GREEN B R,Durnford D G.The chlorophyll-carotenoid proteins of oxygenic photosynthesis[J].Plant Biol,1996(47): 685- 714.
[9]  邰付菊,李 扬,陈 良,等.低温胁迫下棉花子叶蛋白质差异表达的双向电泳分析[J].华中师范大学学报,2008,42(2):262-265. TAI Fu-ju,Li Yang,Chen Liang,et al.Analysis of two-dimensional electrophoresis for the expressed proteins in cotton cotyledons under cold stress[J].Journal of Huazhong Normal University,2008,42(2): 262-265.
[10]  DAY A,Ellis T H N.Chloroplast DNA deletions associated with wheat plant regenerated from pollen: possible basis for maternal inheritance of chloroplasts[J].Cell,1984,39(2): 359- 368.
[11]  孙敬三,吴石君,朱至清,等.水稻白化花粉植株可溶性蛋白质的电泳分析[J].遗传学报, 1977, 4(4):359- 360. SUN Jing-san,Wu Shi-jun,Zhu Zhi-qing,et al. Analysis of soluble proteins in the albino pollen-plants of rice by polyacrylamide gel electrophoresis[J].Acta Genetic Sinica,1977,4(4):359-360.
[12]  杨 炜,钱 前,黄大年.水稻白绿苗可转化性与核酮糖 1,5-二磷酸羧化酶/加氧酶小亚基的关系[J].科学通报,1992(20):1897- 1900. YANG Wei,Qian Qian,Huang Da-nian.The relationship between the transferability of white green rice seedings and ribulose 1,5-diphosphate spindle/oxygenase enzyme small subunit[J].Chinese Science Bulletin,1992(20): 1897-1900.
[13]  许志茹,崔国新,李春雷,等.芜菁黄烷酮3-羟化酶基因的克隆、序列分析及表达[J].分子植物育种,2008,6(4):787- 792. XU Zhi-ru,Cui Guo-xin,Li Chun-lei,et al.Cloning,sequence analysis and expression of flavanone 3-hydroxylase gene in turnip[J].Molecular Plant Breeding,2008,6(4): 787-792.
[14]  ZHONG S W,Zhao M T,Shi T Y,et al.EIN3/EIL1 cooerate with PIF1 to prevent photo-oxidation and to promote greening of Arabidopsis seedings[J].PNAS,2009,106(50): 21431- 21436.
[15]  孔垂华,胡 飞,谢华亮,等.外源多胺对水稻萌发和前期生长的作用及其在土壤中的滞留[J].应用生态学报,1996,7(4):377-380. KONG Chui-hua,Hu Fei,Xie Hua-liang,et al. Effect of supplemented polyamines on the germination and early growth of rice and their adsorption in soil[J]. Chinese Journal of Applied Ecology,1996,7(4): 377-380.
[16]  林文雄,吴杏春,梁康迳,等.UV-B 辐射增强对水稻多胺代谢及内源激素含量的影响[J].应用生态学报,2002,13(7): 807-813. LIN Wen-xiong,Wu Xing-chun,Liang Kang-jing,et al.Effect of enhanced UV-B radiation on polyamine metabolism and endogenous hormone contents in rice (Oryza sativa L.)[J].Chinese Journal of Applied Ecology,2002,13(7): 807-813.
[17]  施木田,陈如凯.锌硼营养对苦瓜产量品质与叶片多胺、激素及衰老的影响[J].应用生态学报,2004,15(1):77-80.SHI Mu-tian,Chen Ru-kai. Effects of zinc and boron nutrition on balsam pear(Momordica charantia) yield and quality,and polyamines,hormone,and senescence of its leaves[J]. Chinese Journal of Applied Ecology,2004,15(1): 77-80.
[18]  樊金萍,柏 锡,李 勇,等.野生大豆S-腺苷甲硫氨酸合成酶基因的克隆及功能分析[J].作物学报,2008,34(9): 1581-1587. FAN Jin-ping,Bo Xi,Li Yong,et al.Cloning and function analysis of gene SAMS from Glycine soja[J].Acta Agronomica Sinica,2008,34(9): 1581-1587.

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