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棉花学报  2013 

雷蒙德氏棉3条染色体特异BAC的筛选与定位

DOI: 1002-7807(2013)04-0323-06, PP. 323-328

Keywords: 雷蒙德氏棉,单染色体,细菌人工染色体,荧光原位杂交

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Abstract:

通过筛选海岛棉BAC文库并结合BAC-FISH技术成功获得雷蒙德氏棉D501、D502和D510染色体的特异BAC克隆,可作为这3条染色体的细胞学标记。这3个克隆在雷蒙德氏棉和海岛棉中进行比较作图,结果表明,它们在海岛棉中均表现出了染色体特异性,说明这3个BAC也可用于海岛棉Db01、Db02和Db10染色体的识别。其中BAC克隆280G06在两棉种10号染色体上的位置为非线性关系,可能在棉属的进化过程中发生了染色体结构的变异。

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