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棉花学报  2013 

棉花精氨酸脱羧酶基因GhADC1克隆与表达分析

DOI: 1002-7807(2013)04-0291-09, PP. 291-299

Keywords: 棉花,精氨酸脱羧酶基因,多胺,黄萎病菌,基因表达

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Abstract:

以抑制性消减杂交(SSH)文库序列为基础,采用生物信息学和RT-PCR方法从陆地棉中克隆出精氨酸脱羧酶基因,命名为GhADC1,GenBank登录号为KC851856。该基因全长2954bp,其中5'非翻译区477bp,具有多个参与胁迫响应的顺式作用元件和一个编码8个氨基酸的微型编码序列(uORF);主编码区(mORF)2181bp,共编码726个氨基酸,其中N端具有叶绿体定位信号肽。推测的GhADC1蛋白具有PLP结合位点及Orn/Dap/Arg脱羧酶的信号位点,属于典型的III型PLP依赖型精氨酸脱羧酶家族成员。荧光定量RT-PCR结果表明,正常条件下,GhADC1在叶片中表达量高于茎和根部组织,且在抗黄萎病品种冀棉20中的表达水平高于耐病品种中521;黄萎病菌胁迫能够快速诱导抗病品种根部GhADC1表达,而在耐病品种中未见明显差异,推测在抗病品种中GhADC1可能参与根部对黄萎病菌胁迫的早期应答。

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