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棉花学报  2013 

外源松柏醛和芥子醛影响棉纤维苯丙烷代谢途径基因表达及生长

DOI: 1002-7807(2013)04-0334-05, PP. 334-338

Keywords: 松柏醛,芥子醛,苯丙烷途径,基因表达,生长

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Abstract:

利用棉花胚珠离体培养和半定量RT-PCR及数字化图像处理技术,通过体外饲喂不同浓度苯丙烷代谢途径中间代谢物松柏醛(CA)和芥子醛(SA)来研究在棉花纤维发育过程中苯丙烷代谢关键中间产物对棉花纤维发育的影响。结果表明,在离体培养棉纤维中,不同浓度(100μmol·L-1、200μmol·L-1)外源松柏醛(CA)和芥子醛(SA)不但诱导了以CA和SA为底物的基因产物表达量增加,也诱导了苯丙烷代谢途径上游基因产物的表达量增加,且高浓度(200μmol·L-1)诱导的表达量高于低浓度(100μmol·L-1),芥子醛高于松柏醛;对GhExpansin1的表达和纤维生长均有抑制作用,且高浓度(200μmol·L-1)抑制作用更加明显,芥子醛抑制作用大于松柏醛。

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