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棉花学报  2013 

一种适宜棉花microRNA实时定量分析的总RNA提取方法

DOI: 1002-7807(2013)05-0467-04, PP. 467-470

Keywords: 棉花,总RNA,提取,microRNA,实时定量

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Abstract:

为满足棉花组织microRNA结构与功能研究的需要,建立了棉花高质量的总RNA提取方法。以陆地棉纤维和胚珠为供试材料,采用改良的异硫氰酸胍-硫氰酸铵-酸酚法提取总RNA,利用stem-loopRT-qPCR法鉴定成熟的miRNA。结果表明此方法能得到高质量的总RNA,并且miRNA的回收率较高,足以满足microRNA水平的实时定量分析。

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