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棉花学报  2013 

风、蜜蜂因素对转Cry1Ac基因棉花花粉介导的基因漂移的影响

DOI: 1002-7807(2013)05-0453-06, PP. 453-458

Keywords: 转Bt基因棉花,基因漂移,花粉介导,安全性评估

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Abstract:

在温室内人工创造风力和释放传粉昆虫蜜蜂的条件下,应用PCR和蛋白试纸条结合的方法检测外源Cry1Ac基因通过花粉漂移至非转基因棉的频率和距离。结果表明风力处理和蜜蜂处理的基因漂移频率均显著高于空白对照。漂移至非转基因亲本棉石远321的频率显著高于陆地棉中棉所35和海岛棉吉扎1号。漂移至石远321的频率随距离远近差异显著,而漂移至中棉所35和吉扎1号的频率在不同距离上差异不显著。风力处理共检测到阳性样本72个,在检测范围内,漂移至石远321的最远距离为25.6m,漂移至中棉所35和吉扎1号的最远距离均为19.2m。蜜蜂处理中共检测到阳性样本75个,在检测范围内,漂移到常规棉的最远漂移距离均达到设置最远处36m,并在此处达到峰值。本研究可为转基因棉花基因漂移生态风险性评估提供参考。

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