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棉花学报  2013 

盐分胁迫下2-氯-6-(三氯甲基)吡啶浸种对棉种发芽及其生理特性的影响

DOI: 1002-7807(2013)05-0426-06, PP. 426-431

Keywords: 2-氯-6-(三氯甲基)吡啶,盐分胁迫,棉种,发芽率,酶活性

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Abstract:

通过设置0、80、120mmol·L-13种NaCl浓度,研究了3种盐分水平下2-氯-6-(三氯甲基)吡啶浸种对棉种发芽的影响,分析了棉种萌发过程中可溶性蛋白、可溶性糖、抗氧化物酶及丙二醛含量对2-氯-6-(三氯甲基)吡啶浸种的生理响应。结果表明,盐分胁迫显著抑制了棉种的萌发,而2-氯-6-(三氯甲基)吡啶浸种能明显减轻盐分胁迫对种子发芽的抑制。在3个盐分水平(0、80、120mmol·L-1)下,2-氯-6-(三氯甲基)吡啶浸种处理的发芽率、发芽势及种子活力指数均比清水浸种有显著提高,其增幅分别为11.5%~58.4%、16.8%~65.0%和42.9%~82.5%;同时,2-氯-6-(三氯甲基)吡啶浸种可显著提高棉种SOD、POD、CAT的活性,分别比清水浸种增加了57.2%~282.7%、8.3%~139.3%和6.4%~15.1%,且显著降低了电解质外渗率与MDA含量。在盐分胁迫下,利用4.1×10-3mmol·L-1的2-氯-6-(三氯甲基)吡啶浸种可提高棉种抗氧化物酶活性,促进其萌发过程中可溶性有机物的转化,显著提高棉种发芽率和发芽势。

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