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棉花学报  2013 

利用基因芯片技术筛选棉花产量性状相关的基因

DOI: 1002-7807(2013)05-0417-09, PP. 417-425

Keywords: 棉花,产量,基因芯片,GhMKRP2,GhPOD

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Abstract:

根据皮棉产量数据的重复性测验及显著性分析,从SG747(GossypiumhirsutumL.)和Giza75(GossypiumBarbadenseL.)的回交自交系群体中选取高产组和低产组品系各3个材料,取这2组材料和其父母本开花后10d的纤维进行芯片分析。比较芯片数据,获得了1508个差异表达基因。对这些差异表达基因进行聚类分析,结果显示高产组品系和低产组品系分别聚类在一起,符合基于田间数据分组的预期结果。利用Blast2GO软件对1508个差异表达基因进行Blast-Mapping-Annotation-KEGG分析,结果表明参与细胞质膜发育、过氧化物酶活性、抗逆和营养物质运输等生物学过程的基因最多。进一步利用RT-PCR分析,得到一系列与纤维品质和产量等性状密切相关的基因。研究结果为棉花高产基因工程和分子育种提供了丰富的基因资源。

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