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棉花学报  2013 

陆地棉MADS-box基因GhMADS13的功能分析

DOI: 1002-7807(2013)05-0377-05, PP. 377-381

Keywords: 陆地棉,MADS-box,GhMADS13,转基因

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Abstract:

为了研究GhMADS13的功能,利用NCBI上提交的序列设计引物进行PCR扩增,扩增序列与提交序列的ORF(Openreadingframe)的一致性为100%。qRT-PCR结果表明棉花的各个组织中,GhMADS13在花中的表达量最高,是表达量低的根的几百倍;花器官中GhMADS13在萼片、花瓣、雄蕊、心皮和胚珠中都有表达,表达量虽有差异,但差异不大,其在胚珠中的表达量最高。将GhMADS13插入到pBI121载体上,构建了植物超表达载体。通过浸花法转化拟南芥,获得了2个转基因株系,分子检测和表型数据统计的结果表明GhMADS13的转录水平越高植株越矮小,角果的长度越短,种子的数目越少。根据GhMADS13的qRT-PCR结果和异位表达分析,推测GhMADS13主要抑制胚珠的发育。

References

[1]  ANGENENT G C,Colombo L.Molecular control of ovule development[J].Trends in Plant Science,1996,1(7): 228-232.
[2]  PELAZ S,Ditta G S,Baumann E,et al.B and C floral organ identity functions require SEPALLATA MADS-box genes[J].Nature,2000,405(6783): 200-203.
[3]  THEIBEN G.Development of floral organ identity: stories from the MADS house[J]. Current Opinion in Plant Biology,2001,4(1): 75-85.
[4]  吴 东,喻树迅,范术丽,等.棉花 MADS-box 蛋白基因 (GhMADS-13) 的克隆和表达分析[J]. 基因组学与应用生物学,2009,28(2):223-228. WU Dong,Yu Shu-xun,Fan Shu-li,et al.Cloning and expression analysis of a MADS-box protein gene (GhMADS-13) from upland cotton[J].Genomics and Applied Biology,2009,28(2): 223-228.
[5]  MA H,Yanofsky M F,Meyerowitz E M.AGL1-AGL6,an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes[J].Genes & Development,1991,5(3): 484-495.
[6]  ALVAREZ-Buylla E R,Pelaz S,Liljegren S J,et al.An ancestral MADS-box gene duplication occurred before the divergence of plants and animals[J].Proceedings of the National Academy of Sciences of USA,2000,97(10): 5328-5333.
[7]  CLOUGH S J,Bent A F.Floral dip: a simplified method for Agrobacterium‐mediated transformation of Arabidopsis tha- liana[J].The Plant Journal,1998,16(6): 735-743.
[8]  KOO S C,Bracko O,Park M S,et al.Control of lateral organ development and flowering time by the Arabidopsis thaliana MADS‐box Gene AGAMOUS‐LIKE6[J].The Plant Journal,2010,62(5): 807-816.
[9]  HSU H F,Huang C H,Chou L T,et al.Ectopic expression of an orchid (Oncidium Gower Ramsey) AGL6-like gene promotes flowering by activating flowering time genes in Arabidopsis thaliana[J].Plant and Cell Physiology,2003,44(8): 783-794.
[10]  FAN Jin-hui,Li Wen-qiong,Dong Xin-chun,et al.Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis[J].Science in China Series C: Life Sciences,2007,50(5): 676-689.
[11]  MESSENGUY F,Dubois E.Role of MADS box proteins and their cofactors in combinatorial control of gene expression and cell development[J].Gene,2003,316(1): 1-21.
[12]  COEN E S,Meyerowitz E M.The war of the whorls: genetic interactions controlling flower development[J].Nature,1991,353(6339): 31-37.
[13]  SCHWARZ-SOMMER Z,Huijser P,Nacken W,et al.Genetic control of flower development by homeotic genes in Antirrhinum majus[J].Science,1990,250(4983): 931-936.
[14]  BOWMAN J L,Smyth D R,Meyerowitz E M.Genetic interactions among floral homeotic genes of Arabidopsis[J].Development,1991,112(1): 1-20.
[15]  WEIGEL D,Meyerowitz E M.The ABCs of floral homeotic genes[J].Cell,1994,78(2): 203-209.

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