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棉花学报  2014 

陆地棉种子发育过程中microRNA的挖掘与功能研究

DOI: 1002-7807(2014)01-0081-06, PP. 81-86

Keywords: 陆地棉,基因芯片,miRNA,茎环RT-PCR

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Abstract:

microRNAs(miRNAs)广泛参与调控植物的生长和发育,但这类分子是否对棉花胚珠和纤维的生长发育起到重要的作用,还有待验证。对这些小分子的认识,一般先要从序列信息的获得上开始。应用基因芯片对TM-1+5DPA(开花后5d)胚珠总RNA进行了miRNA的筛选研究。结果显示,含有352个探针的芯片成功钓取到199个陆地棉miRNA,其中绝大多数为首次报道。此外,对miR399研究发现,它与纤维中磷的含量变化相关;miR169可能与干旱应激反应有关。

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