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棉花学报  2014 

光质对离体诱导棕色棉纤维基因表达影响的cDNA-SRAP分析

DOI: 1002-7807(2014)01-0034-07, PP. 34-40

Keywords: 离体培养,cDNA-SRAP,谷胱甘肽转移酶,植物抗逆性

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Abstract:

以离体培养棕色棉纤维(暗培养10d)为材料,分别用红、黄、蓝、白光进行处理,在24h光照条件下培养10d后,利用cDNA-SRAP技术对其基因表达进行分析。对其中16个差异片段测序后进行同源性比对和功能分析表明2个序列功能与植物抗逆性有关(CNGC5类似蛋白基因、(+)-δ-杜松烯合成酶);3个序列与生物代谢相关(β-1,3-葡聚糖酶13基因、质膜胆碱转运蛋白基因、tau类谷胱甘肽转移酶基因)。其中,谷胱甘肽转移酶基因与原花青素合成前体物质的转运有关,光质可能通过影响谷胱甘肽转移酶基因进而对原花青素的合成产生影响。

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