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棉花学报  2008 

大丽轮枝菌拮抗细菌的分离与抗菌物质鉴定

DOI: 1002-7807(2008)06-0442-05, PP. 442-446

Keywords: 棉花黄萎病,拮抗细菌,分离,抗菌物,鉴定

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Abstract:

从黄萎病发病棉田未发病棉株根际土样中分离芽孢杆菌,利用对峙培养法对大丽轮枝菌进行平板拮抗实验,筛选出6株对大丽轮枝菌有拮抗作用的芽孢杆菌,其中3株菌发酵代谢产物具有抗菌活性。S4-3菌株发酵液滤液经100℃、30min热处理丧失抗菌活性,发酵液氯仿抽提物不具有抗菌活性,发酵液硫酸铵沉淀物有抑菌活性,但经蛋白酶处理后活性丧失,认为S4-3菌株抑菌活性物质是蛋白质。而菌株S4-5发酵液经100℃热处理后还有较高的抗菌活性,发酵液氯仿抽提物也具有抗菌活性,但硫酸铵沉淀物对病原菌无抑制作用;S5-6菌株发酵液滤液经100℃热处理丧失抗菌活性,硫酸铵沉淀物也不具有抗菌活性,而发酵液氯仿抽提物有抗菌活性,因此认为S4-5和S5-6菌株产生的抑菌物质不是蛋白质类。

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