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棉花学报  2011 

绿盲蝽刺吸胁迫诱导棉花SSH文库的初步构建及生物信息学分析

DOI: 1002-7807(2011)02-0134-06, PP. 134-139

Keywords: 绿盲蝽,刺吸胁迫诱导,SSH文库,生物信息

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Abstract:

为了筛选棉花中对绿盲蝽为害产生防御反应的基因,初步构建了绿盲蝽刺吸胁迫诱导棉花抑制性消减杂交正向文库,构建文库过程中随机挑取960个克隆进行菌落PCR鉴定,插入片段在250~1500bp之间。经反向Northern筛选,测序拼接后得到78个非重复独立基因(unigenes)。通过Blast2GO软件对unigenes进行序列比对,并对差异基因进行功能分类,结果表明,由绿盲蝽刺吸胁迫诱导的棉花应激反应过程包括细胞通讯、酶活性调节、对外界生物和非生物胁迫的应激反应、次级代谢、能量合成代谢以及细胞生长发育等很多生物过程。这些生物过程的变化直接或间接对棉花本身起到一定的保护作用。

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