全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
棉花学报  2011 

棉花八氢番茄红素脱氢酶GhPDS1基因的克隆与表达谱分析

DOI: 1002-7807(2011)03-0200-05, PP. 200-204

Keywords: 棉花,八氢番茄红素脱氢酶,GhPDS1基因,RACE技术,序列分析,表达谱

Full-Text   Cite this paper   Add to My Lib

Abstract:

八氢番茄红素脱氢酶(PDS)在胡萝卜素生理代谢和病毒诱导外源基因沉默中起着非常重要的作用。本研究通过同源克隆方法,分析已报道植物八氢番茄红素脱氢酶氨基酸保守区,设计简并引物,扩增出编码棉花PDS基因cDNA中间片段。通过RACE技术成功地克隆了棉花PDS基因的全长cDNA,命名为GhPDS1(GenBankNo.HQ441184)。利用半定量RT-PCR技术进行组织表达模式分析发现,该基因在花中表达量最高,其次为叶片。棉花PDS基因的成功克隆使得该基因可在病毒诱导的基因沉默研究中作为一个检测显示基因,它的沉默直接导致棉花叶片表现出斑驳、褪色等生物学特征,便于棉花中重要功能基因的深入研究。

References

[1]  王 飞.甘薯类胡萝卜素合成酶基因PDS全长cDNA的克隆[J].安徽理工大学学报:自然科学版,2007,27(001):55-58.
[2]  WANG Fei. Clone of full-length cDNA of phytoene desaturase(Gene pds) Gene in sweet potato(Ipomoea Batatas L.)[J]. Journal of Anhui University of Science and Technology: Natural Science,2007, 27(001): 55-58.
[3]  胡根海,喻树迅.棉花基因克隆研究进展[J].棉花学报,2005,17(4):240-244.
[4]  HU Gen-hai, Yu Shu-xun. Advances in cotton gene cloning[J]. cotton science, 2005, 17(4): 240-244.
[5]  ROTENBERG D, Thompson T S, German T L, et al. Methods for effective real-time RT-PCR analysis of virus-induced gene silencing[J]. Journal of Virological Methods, 2006, 138(1-2): 49-59.
[6]  李兴芳,董雅娟,柏学进,等.5-Aza诱导BMSC 分化为心肌细胞的分子生物学检测[J]. 中国农业科学,2009,42(7): 2539- 2545.
[7]  LI Xing-fang, Dong Ya-juan, Bai Xue-jin. Molecular Biology Detection of 5-Aza Inducing BMSC into Cadiocyte[J]. Scientia Agricultura Sinica, 2009, 42(7): 2539-2545.
[8]  李纪锁,沈火林,石正强.番茄红素的研究进展[J].中国蔬菜,2003,44(1):58-60.
[9]  LI Ji-suo, Shen Huo-lin, Shi Zheng-qiang. Advances on lycopene[J]. China Vegetables,2003, 44(1): 58-60.
[10]  YE Xu-dong, Al-babili S, Kloti A, et al. Engineering the provitamin A (-carotene) biosynthetic pathway into(carotenoid-free) rice endosperm[J]. Science, 2000, 287(5451): 303-305.
[11]  郭新伦.牛奶子果实成熟过程中类胡萝卜素组成及代谢酶基因的克隆与表达[D].杭州:浙江师范大学,2009.
[12]  GUO Xin-lun. Carotenoid composition and cloning and expression analysis of carotenogenic genes during ripening of autumn olive fruit[D]. Hangzhou: Zhejiang Normal University, 2009.
[13]  陈端芬,彭镇华,高志民.中国水仙八氢番茄红素脱氢酶基因(PDS)的克隆及表达分析[J].分子植物育种,2008,6(3):574- 578.
[14]  CHEN Duan-fen, Peng Zhen-hua, Gao Zhi-min. Cloning and expression analysis of PDS gene in Narcissus tazetta var.chinensis[J]. Molecular Plant Breeding, 2008, 6(3): 574-578.
[15]  FOFANA I B F, Sangare A, Collier R, et al. A geminivirus-induced gene silencing system for gene function validation in cassava[J]. Plant Molecular Biology, 2004, 56(4): 613-624.
[16]  朱跃辉,房科腾,姜建国.杜氏藻类胡萝卜素的最佳提取条件的研究及比较[J].广州食品工业科技,2005,31(009):21-23.
[17]  ZHU Yue-hui, Fang Ke-teng, Jiang Jian-guo. Studies on the optimum extraction conditions of carotenoids from Dunaliella[J]. Guangzhou food science and technology, 2005, 31(009): 21-23.
[18]  BURKHARDT P K, Beyer P, Wunn J, et al. Transgenic rice(Oryza sativa) endosperm expressing daffodil(Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis[J]. The Plant Journal, 1997, 11(5): 1071-1078.
[19]  SIMKIN A J, Laboure A M, Kuntz M, et al. Comparison of carotenoid content,gene expression and enzyme levels in tomato(Lycopersicon esculentum) leaves[J]. Zeitschrift Fur Naturforschung, 2003, 58(5/6): 371-380.
[20]  李永平.草莓类胡萝卜素合成相关基因的克隆[D].福州:福建农林大学,2009.
[21]  LI Yong-ping. Cloning of genes for partial member enzumes of carotenoid biosynthetic pathway in strawberry(Fragaria ananassa Duch)[D]. Fuzhou: Fujian Agriculture and Forestry University, 2009.
[22]  BARTLEY G E, Scolnik P A. Plant carotenoids:pigments for photoprotection, visual attraction,and human health[J]. The Plant Cell, 1995, 7(7): 1027-1038.
[23]  JOHN M E, Crow L J. Gene expression in cotton(Gossypium hirsutum L.) fiber: cloning of the mRNAs[J]. Proceedings of the National Academy of Sciences of the United States of America,1992, 89(13): 5769-5773.
[24]  FRASER P D, Romer S, Shipton C A, et al. Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(2): 1092-1097.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133