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棉花学报  2011 

新陆早32号、33号的体细胞胚胎发生和植株再生比较研究

DOI: 1002-7807(2011)03-0259-06, PP. 259-264

Keywords: 棉花,体细胞胚胎,植株再生

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Abstract:

以新疆主栽品种新陆早32号、33号和对照YZ1为研究材料,通过不同浓度的激素组合成功诱导获得了体细胞胚,并进一步发育成苗。研究发现,所用的12种激素组合均能有效诱导愈伤组织。虽然IBA+KT组合有利于诱导YZ1和新陆早33号的快速分化,但在增殖生长过程中体细胞胚容易褐化、死亡;在2,4-D+KT组合中,0.1mg·L-12,4-D+0.1mg·L-1KT诱导新陆早33号的分化速度较快;新陆早32号在高比例2,4-D/KT激素组合(0.1mg·L-12,4-D+0.2mg·L-1KT和0.2mg·L-12,4-D+0.5mg·L-1KT)促进下分化较快;新陆早33号的分化速度和胚胎发生的速度快于新陆早32号。对照品种YZ1下胚轴在低浓度激素组合甚至不加激素的情况下都能快速分化,在高浓度KT下容易褐化、死亡。3个材料在8个月内都成功得到再生苗。

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