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棉花学报  2011 

棉花早熟芽黄突变体叶绿素荧光动力学特性研究

DOI: 1002-7807(2011)05-0414-08, PP. 414-421

Keywords: 棉花,芽黄突变体,叶绿素荧光,光系统Ⅱ

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Abstract:

以中棉所58及其航天诱变芽黄突变体为研究对象,利用快速叶绿素荧光诱导动力学测定和JIP-test数据分析方法,研究了晴天条件下野生型(Wildtype,WT)和突变体5个叶位叶片(倒1叶至倒5叶)的原初光化学反应的变化。结果表明,突变体倒2叶最大光化学效率(Fv/Fm)最低,至倒5叶时恢复正常。突变体叶片较高的K点的相对可变荧光值(Wk)表明放氧复合体受到损害。与野生型相比,突变体新生叶片的O-J-I-P荧光诱导曲线的初始斜率(Mo)升高,标准化后的O-J-I-P荧光诱导曲线、最大荧光强度及y轴之间的面积(Sm)、用于电子传递的量子产额(φEo)、反应中心捕获的激子中用来推动电子传递到电子传递链中超过QA-的其它电子受体的激子占用来推动QA-还原激子的比率(ψo)值降低,表明叶片发育早期PSⅡ受体侧的QA-大量积累,电子传递链受阻。通过分析突变体光合机构的比活性参数发现,在叶片发育的早期,突变体单位反应中心吸收的较多的能量以热和荧光的形式被耗散掉。突变体新叶发育前期黄化、后期变绿,推测早期叶绿素合成受阻,造成光系统损伤、光合性能下降。

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